Dengue and chikungunya viruses in plasma are effectively inactivated after treatment with methylene blue and visible light
Dengue and chikungunya viruses in plasma are effectively inactivated after treatment with methylene blue and visible light
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DOI：
10.1111/trf.13729
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发表时间：
2016-09-01
期刊：
影响因子：
2.9
通讯作者：
Faddy, Helen M.
中科院分区：
文献类型：
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作者：
Fryk, Jesse J.;Marks, Denese C.;Faddy, Helen M.
背景：虫媒病毒，例如登革热病毒（DENV）和基孔肯雅病毒（CHIKV），对包括血浆在内的血液成分的安全输注构成风险。病原体灭活是管理这种输血传播风险的一种方法，世界范围内使用了多种技术来处理血浆。在本研究中，研究了使用亚甲蓝和 630 nm 光照射，THERAFLEX MB-Plasma 系统灭活血浆中所有 DENV 血清型（DENV-1、DENV-2、DENV-3、DENV-4）或 CHIKV 的功效。 研究设计和方法：混合血浆单元中掺入 DENV-1、DENV-2、DENV-3、DENV-4 或CHIKV 并使用 THERAFLEX MB-Plasma 系统以四种光照射剂量进行处理：20、40、60 和 120（标准剂量）J/cm(2)。收集治疗前和治疗后的样本并测定病毒感染性。计算每个剂量的病毒感染性降低。结果：用 THERAFLEX MB-Plasma 系统处理血浆导致所有 DENV 血清型和 CHIKV 感染性病毒至少减少 4.46 个对数。每种残留感染性均处于 60 J/cm 2 检测极限，并且还观察到剂量依赖性。 结论：我们的研究表明，THERAFLEX MB-Plasma 系统可以将所有 DENV 血清型和掺入血浆的 CHIKV 的感染性降低至标准照明剂量一半时使用的检测极限。这表明该系统有能力成为管理血浆中 DENV 或 CHIKV 输血传播风险的有效选择。
BACKGROUND: Arboviruses, such as dengue viruses (DENV) and chikungunya virus (CHIKV), pose a risk to the safe transfusion of blood components, including plasma. Pathogen inactivation is an approach to manage this transfusion transmission risk, with a number of techniques being used worldwide for the treatment of plasma. In this study, the efficacy of the THERAFLEX MB-Plasma system to inactivate all DENV serotypes (DENV-1, DENV-2, DENV-3, DENV-4) or CHIKV in plasma, using methylene blue and light illumination at 630 nm, was investigated.STUDY DESIGN AND METHODS: Pooled plasma units were spiked with DENV-1, DENV-2, DENV-3 DENV-4, or CHIKV and treated with the THERAFLEX MB-Plasma system at four light illumination doses: 20, 40, 60, and 120 (standard dose) J/cm(2). Pre- and posttreatment samples were collected and viral infectivity was determined. The reduction in viral infectivity was calculated for each dose.RESULTS: Treatment of plasma with the THERAFLEX MB-Plasma system resulted in at least a 4.46-log reduction in all DENV serotypes and CHIKV infectious virus. The residual infectivity for each was at the detection limit of the assay used at 60 J/cm 2, with dose dependency also observed.CONCLUSIONS: Our study demonstrated the THERAFLEX MB-Plasma system can reduce the infectivity of all DENV serotypes and CHIKV spiked into plasma to the detection limit of the assay used at half of the standard illumination dose. This suggests this system has the capacity to be an effective option for managing the risk of DENV or CHIKV transfusion transmission in plasma.