Karrikins-induced accumulation of Salvianolic acid B is regulated by Jasmonic acid and hydrogen peroxide in Salvia miltiorrhiza
Karrikins-induced accumulation of Salvianolic acid B is regulated by Jasmonic acid and hydrogen peroxide in Salvia miltiorrhiza
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DOI：
10.1016/j.sajb.2020.01.021
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发表时间：
2020-05
影响因子：
3.1
通讯作者：
Hui Sun;Xu Zixin-;Jie Zhou;Lanping Guo
中科院分区：
文献类型：
--
作者：
Hui Sun;Xu Zixin-;Jie Zhou;Lanping Guo
本文研究了烟熏分离的Karrikins（KAR 1）对丹参内源信号分子和次生代谢产物丹参素B（SA B）的影响。这些结果表明，KAR 1诱导茉莉酸（JA）和过氧化氢（H2 O2）的积累以及SAB的产生增加。KAR 1处理提高了JA和H2 O2的含量，表明JA和H2 O2的产生是S.在本实验中，丹参对KAR 1有反应。JA抑制剂可抑制KAR 1诱导的JA和H2 O2的产生，表明KAR 1诱导的JA积累位于H2 O2合成的上游。JA特异性清除剂水杨酸异羟肟酸（SHAM）和没食子酸正丙酯（PrGall）以及H2 O2生物合成抑制剂过氧化氢酶（CAT）和二苯碘鎓（DPI）均抑制KAR 1诱导的SAB积累，表明JA和H2 O2在KAR 1诱导的SAB积累中起信号作用。CAT/DPI可抑制外源JA诱导的KAR 1对SAB的促进作用。此外，H2 O2可逆转KAR 1诱导的SHAM、PrGall对SAB产生的抑制作用。结果表明JA介导KAR 1诱导的SAB增加依赖于H2 O2 inS的产生。丹参。
The influence of smoke-isolated karrikins (KAR1) on the production of endogenous signal molecules and secondary metabolite Salvianolic acid B (SAB) inSalvia miltiorrhizahas been studied. These results indicated that KAR1induced increase of the accumulation of jasmonic acid (JA) and hydrogen peroxide (H2O2) as well as SAB production. The contents of JA and H2O2were increased by treatment of KAR1, suggesting that production of JA and H2O2was early biochemical reaction ofS. miltiorrhizaresponding to KAR1in this experiment. KAR1-stimulated generation of JA and H2O2was inhibited by JA inhibitors, which indicated that KAR1-induced accumulation of JA was located in the upstream of H2O2biosynthesis. Treatments with JA-specific scavengers (salicylhydroxamic acid (SHAM) and n-propylgallate (PrGall)) and H2O2biosynthesis inhibitors (catalase (CAT) and diphenylene iodonium (DPI)) inhibited KAR1-induced accumulation of SAB, which suggested that JA and H2O2played signal roles in KAR1-stimulated accumulation of SAB. CAT/DPI could inhibit KAR1-induced promotion of SAB induced by exogenous JA. In addition, application of H2O2could reverse KAR1-induced inhibitory effects of SHAM, PrGall toward SAB production. Our results indicated that JA mediated the KAR1-induced increase of SAB dependent on the generation of H2O2inS. miltiorrhiza.