Cholecystokinin octapeptide inhibits the in vitro expression of CD14 in rat pulmonary interstitial macrophages induced by lipopolysaccharide.
Cholecystokinin octapeptide inhibits the in vitro expression of CD14 in rat pulmonary interstitial macrophages induced by lipopolysaccharide.
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发表时间：
2002-02
影响因子：
6.1
通讯作者：
Shujin Li;B. Cong;Yu-xian Yan;Yuxia Yao;Chunling Ma;Y. Ling
中科院分区：
文献类型：
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作者：
Shujin Li;B. Cong;Yu-xian Yan;Yuxia Yao;Chunling Ma;Y. Ling
目的研究八肽胆囊收缩素（CCK-8）对脂多糖（LPS）刺激的肺间质巨噬细胞（PIM）的影响。方法分离培养PIM，分别在LPS、CCK-8、CCK受体拮抗剂丙谷胺和溶剂存在或不存在的条件下培养。流式细胞术检测细胞膜CD 14（mCD 14）蛋白的表达，Western blot半定量分析细胞培养上清中可溶性CD 14（sCD 14）的含量。用ELISA检测上清液中的TNF-α。结果CCK-8在10（-7）mol/L和10（-6）mol/L浓度下可显著抑制mCD 14的表达。LPS（1 μ g/ml）可上调上清液中sCD 14和TNF-α的释放，但CCK-8可降低其释放。丙谷胺可抑制CCK-8的作用。结论CCK-8通过CCK受体负性调节LPS刺激的PIM的多种功能。这可能是CCK-8减轻内毒素血症时肺组织炎症反应的机制之一。
OBJECTIVE To study the effect of cholecystokinin octapeptide (CCK-8) on lipopolysaccharide (LPS)-stimulated pulmonary interstitial macrophages (PIM) in vitro. METHODS PIM were isolated and cultured in the presence or absence of LPS, CCK-8, proglumide (the antagonist of CCK receptors) and vehicle. The expression of membrane CD14 (mCD14) protein was assayed by flow cytometry and soluble CD14 (sCD14) in the supernatant was analyzed semi-quantitatively by Western blot. TNF-alpha in the supernatant was detected with ELISA. RESULTS CCK-8, at concentrations of 10(-7) mol/L and 10(-6) mol/L, significantly inhibited the expression of mCD14. Release of sCD14 and TNF-alpha in the supernatant was up-regulated by LPS (1 microg/ml) but reduced by CCK-8. The effect of CCK-8 was inhibited by proglumide. CONCLUSION CCK-8 negatively modulated several functions of LPS-stimulated PIM through CCK receptors. This may be one of the mechanisms for CCK-8 to alleviate inflammation in lung tissue during endotoxemia.