GFP vectors for controlled expression and dual labelling of protein fusions in Bacillus subtilis
GFP vectors for controlled expression and dual labelling of protein fusions in Bacillus subtilis
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DOI：
10.1016/s0378-1119(98)00580-0
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发表时间：
1999-02-04
期刊：
影响因子：
3.5
通讯作者：
Marston, AL
中科院分区：
文献类型：
--
作者：
Lewis, PJ;Marston, AL
我们报道了一系列质粒载体的开发，用于构建与固有荧光绿色荧光蛋白突变体的融合体，GFPmut1 (Cormack et al., 1996. Gene 173, 33-38) 和 GFPuv (Crameri et al., 1996. Nature Biotechnology 14, 315-319)。可以产生 N 端和 C 端融合体，并且在双交换整合到枯草芽孢杆菌染色体的 amyE 基因座后，可以通过诱导型 Pxyl 启动子精细控制它们的表达。其他设计用于单交换插入染色体的载体允许将下游基因置于诱导控制之下。我们还表明，由于 GFPmut1 和 GFPuv 的激发光谱不同，它们的融合可以在细胞内共定位。 (C) 1999 Elsevier Science B.V. 保留所有权利。
We report the development of a series of plasmid vectors for the construction of fusions to mutants of the intrinsically fluorescent green fluorescent protein, GFPmut1 (Cormack et al., 1996. Gene 173, 33-38) and GFPuv (Crameri et al., 1996. Nature Biotechnology 14, 315-319). Both N- and C-terminal fusions can be produced, and their expression can be finely controlled from the inducible Pxyl promoter following double crossover integration into the amyE locus of the Bacillus subtilis chromosome. Other vectors designed for single crossover insertion into the chromosome allow downstream genes to be placed under inducible control. We also show that fusions to GFPmut1 and GFPuv can be co-localized within the cell by virtue of their different excitation spectra. (C) 1999 Elsevier Science B.V. All rights reserved.