Combination of exercise and enalapril enhances renoprotective and peripheral effects in rats with renal ablation
Combination of exercise and enalapril enhances renoprotective and peripheral effects in rats with renal ablation
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DOI：
10.1016/j.amjhyper.2005.07.009
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发表时间：
2006-01-01
影响因子：
3.2
通讯作者：
Kohzuki, M
中科院分区：
文献类型：
--
作者：
Kanazawa, M;Kawamura, T;Kohzuki, M
背景：有研究表明，适当的慢性运动(EX)可以改善慢性肾功能衰竭(CRF)患者的体力。由于急性运动可引起蛋白尿，降低肾血流量和肾小球滤过率，因此有必要考虑运动对肾功能的影响。方法：雄性5/6肾切除大鼠分为6组：1)不运动组(C)，2)ENA组(2 mg/kg/d，皮下注射)，3)中度运动+跑台运动(20 m/min，60 min/d，5天/周)(Exm组)，3)中度运动+跑台跑(60 m/d，5天/周)，5天/周，5天/周。4)大强度运动+跑台跑(28m/min，每天60min，每周5天)(EXi)；(5)EXM+ENA；(6)假手术(S)。结果：EX和ENA均能抑制高血压的发展，减缓蛋白尿的增加，降低血肌酐和血尿素氮，改善肾小球硬化指数(IGS)和肾皮质相对间质体积(RIV)。此外，在所有其他肾切除组中，EXM+ENA组的IGS和RIV最低。此外，Exm+ENA可促进比目鱼肌毛细血管形成和I型纤维的比例。结论：EX和ENA具有肾脏保护作用。研究结果还表明，在这种大鼠模型中，EXM+ENA提供了比单独ENA更强的肾脏保护作用，并且在没有任何并发症的情况下，EXM+ENA具有一些额外的外周效应。
Background: It is suggested that appropriate chronic exercise (EX) may produce improvements of the physical strength in patients with chronic renal failure (CRF). Because acute exercise causes proteinuria and decreases the renal blood flow and glomerular filtration rate, it is necessary to consider the influence of EX on renal function. Therefore, we assessed the renal and peripheral effects of moderate to intense EX as well as the effects of the combination of EX and enalapril (ENA) in a rat model of CRF.Methods: Male 5/6-nephrectomized Wistar-Kyoto rats were divided into six groups according to the following treatment: 1) no exercise (C); 2) ENA (2 mg/kg/day, subcutaneously); 3) moderate exercise with treadmill running (20 m/min for 60 min/day, 5 days/week) (EXm); 4) intense exercise with treadmill running (28 m/min for 60 min/day, 5 days/week) (EXi); 5) EXm+ENA; and 6) sham operation (S). The rats were then treated for 12 weeks.Results: Both EX and ENA blocked the development of hypertension, blunted increases in proteinuria, reduced serum creatinine and blood urea nitrogen, and improved the index of glomerular sclerosis (IGS) and the relative interstitial volume of the renal cortex (RIV). Moreover, IGS and RIV in the EXm+ENA group were the lowest among all other nephrectomized groups. Furthermore, EXm+ENA enhanced capillarization as well as the proportion of type-I fiber in the soleus muscle.Conclusions: These results suggest that EX and ENA have renoprotective effects. The findings also suggest that EXm+ENA provided greater renoprotective effects than those of ENA alone, and that EXm+ENA had some additional peripheral effects without any complications in this rat model.