Neural stem cells and neurospheres - re-evaluating the relationship
Neural stem cells and neurospheres - re-evaluating the relationship
复制标题
DOI：
10.1038/nmeth758
复制
发表时间：
2005-05-01
期刊：
影响因子：
48
通讯作者：
Rietze, RL
中科院分区：
文献类型：
--
作者：
Reynolds, BA;Rietze, RL
在上个世纪的大部分时间里，由于认为成年哺乳动物的中枢神经系统(CNS)不能产生新的神经细胞，取代丢失或受损的中枢神经系统(CNS)细胞的前景受到阻碍。与大多数教条一样，这种信念基本上是建立在缺乏实验证据的基础上的。这一“无新神经元”假说的推翻始于20世纪中叶的一系列报告，记录了出生后和成年脑中的神经发生(1)，继续从成年哺乳动物脑中分离和体外培养神经源性细胞(2，3)，最终在成年中枢神经系统(即真正的神经干细胞)中发现了一群多能的自我更新细胞(3-5)。尽管最初使用了各种技术，但神经球试验(NSA)(3，6)迅速成为选择的分析方法，并已成为分离和了解胚胎和成人中枢神经干细胞生物学的有价值的工具。与所有技术一样，它也不是没有局限性。在这篇文章中，我们将强调与其应用和解释相关的分析的几个缺陷，我们认为这些缺陷导致了大量的研究，其结论很可能具有误导性。
For most of the past century, the prospect of replacing lost or damaged cells in the central nervous system (CNS) was hampered by the opinion that the adult mammalian CNS was incapable of generating new nerve cells. This belief, Like most dogmas, was essentially founded on a lack of experimental evidence to the contrary. The overturning of this 'no new neuron' hypothesis began midway through the twentieth century with a series of reports documenting neurogenesis in the postnatal and adult brain(1), continued with the isolation and in vitro culture of neurogenic cells from the adult mammalian brain(2,3), and culminated in the discovery of a population of muttipotent, selfrenewing cells in the adult CNS (that is, bona fide neural stem cells)(3-5). Although a variety of techniques were initially used, the neurosphere assay (NSA)(3,6) rapidly emerged as the assay of choice and has since become a valuable toot for isolating, and understanding the biology of, embryonic and adult CNS stem cells. Like all technologies, it is not without its limitations. In this article we will hightight several shortcomings of the assay related to its application and interpretation that we believe have led to a significant body of research whose conclusions may well be misleading.