The genetic architecture of odor-guided behavior in Drosophila:: epistasis and the transcriptome
The genetic architecture of odor-guided behavior in Drosophila:: epistasis and the transcriptome
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DOI：
10.1038/ng1240
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发表时间：
2003-10-01
期刊：
影响因子：
30.8
通讯作者：
Mackay, TFC
中科院分区：
文献类型：
--
作者：
Anholt, RRH;Dilda, CL;Mackay, TFC
我们结合转录谱分析和定量遗传分析来阐明果蝇嗅觉行为的遗传结构。我们应用全基因组表达分析五个共基因嗅觉受损（smi）突变系和他们的控制。我们使用方差分析来划分男性和女性之间以及smi基因型之间的转录本丰度的变化，并确定基因型与性别的相互作用。共有666个基因表现出转录丰度的性别二型性，530个基因在一个或多个smi突变的反应中被共调节，在转录组水平上表现出相当大的上位性。这些共调节基因的突变与smi突变的定量互补测试表明，在大多数情况下（67%），嗅觉行为的上位相互作用反映了转录水平上的上位性，从而识别出调节嗅觉行为的新候选基因。
We combined transcriptional profiling and quantitative genetic analysis to elucidate the genetic architecture of olfactory behavior in Drosophila melanogaster. We applied whole-genome expression analysis to five coisogenic smell-impaired (smi) mutant lines and their control. We used analysis of variance to partition variation in transcript abundance between males and females and between smi genotypes and to determine the genotype-by-sex interaction. A total of 666 genes showed sexual dimorphism in transcript abundance, and 530 genes were coregulated in response to one or more smi mutations, showing considerable epistasis at the level of the transcriptome in response to single mutations. Quantitative complementation tests of mutations at these coregulated genes with the smi mutations showed that in most cases (67%) epistatic interactions for olfactory behavior mirrored epistasis at the level of transcription, thus identifying new candidate genes regulating olfactory behavior.