N-acylhomoserine lactone-regulation of genes mediating motility and pathogenicity in Pseudomonas syringae pathovar tabaci 11528.
N-acylhomoserine lactone-regulation of genes mediating motility and pathogenicity in Pseudomonas syringae pathovar tabaci 11528.
复制标题
N-酰基高丝氨酸内酯调节介导丁香假单胞菌烟粉虱运动性和致病性的基因 11528
DOI：
10.1002/mbo3.440
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发表时间：
2017-06
期刊：
影响因子：
3.4
通讯作者：
Zhuang G
中科院分区：
文献类型：
--
作者：
Cheng F;Ma A;Luo J;Zhuang X;Zhuang G
烟草假单胞菌致病变种11528（Pseudomonaserichingae pathovar tabaci 11528）是一种在大豆和烟草植物中引起野火病的植物病原体。它利用了一种称为群体感应（QS）的细胞密度依赖性调节系统。在其QS系统中，psyI负责N-酰基高丝氨酸内酯（AHL）的生物合成。通过使用RNA测序（RNA-seq）技术比较来自P. lingae 11528野生型菌株与ΔpsyI突变体的转录物，在从指数生长期到稳定生长期的过渡中鉴定了1118个阿勒调控基因。许多阿勒调控的致病基因受到负调控，包括与鞭毛、趋化性、菌毛、胞外多糖、分泌系统和双组分系统相关的基因。此外，基因本体和途径富集分析显示，最明显的调节与细菌运动性有关。最后，表型分析表明，QS调节的性状参与了病原体的附生生长和植物的疾病发展。这些发现意味着P. lingae 11528中阿勒介导的QS系统在植物和病原体之间相互作用的不同阶段发挥重要作用，包括早期植物定殖和晚期植物感染。
Pseudomonas syringae pathovar tabaci 11528 (P. syringae 11528) is a phytopathogen that causes wild‐fire disease in soybean and tobacco plants. It utilizes a cell density‐dependent regulation system known as quorum sensing (QS). In its QS system, the psyI is responsible for the biosynthesis of N‐acylhomoserine lactones (AHLs). By comparing the transcripts from P. syringae 11528 wild‐type strain with those of the ΔpsyI mutant using RNA sequencing (RNA‐seq) technology, 1118 AHL‐regulated genes were identified in the transition from exponential to stationary growth phase. Numerous AHL‐regulated genes involved in pathogenicity were negatively controlled, including genes linked to flagella, chemotaxis, pilus, extracellular polysaccharides, secretion systems, and two‐component system. Moreover, gene ontology and pathway enrichment analysis revealed that the most pronounced regulation was associated with bacterial motility. Finally, phenotypic assays showed that QS‐regulated traits were involved in epiphytic growth of pathogens and disease development in plants. These findings imply that the AHL‐mediated QS system in P. syringae 11528 plays significant roles in distinct stages of interactions between plants and pathogens, including early plant colonization and late plant infection.