A synthetic A tail rescues yeast nuclear accumulation of a ribozyme-terminated transcript
A synthetic A tail rescues yeast nuclear accumulation of a ribozyme-terminated transcript
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DOI：
10.1261/rna.7166704
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发表时间：
2004-12-01
期刊：
影响因子：
4.5
通讯作者：
Rosbash, M
中科院分区：
文献类型：
--
作者：
Dower, K;Kuperwasser, N;Rosbash, M
为了研究 3' 末端形成在酵母 mRNA 输出中的作用，我们用自切割锤头核酶元件替换了 mRNA 切割和聚腺苷酸化信号。产生的 RNA 是未腺苷酸化的，并在其合成位点附近积累。尽管如此，该 RNA 的很大一部分到达了细胞质。通过在核酶元件（合成的 A 尾）紧邻上游插入一段 DNA 编码的腺苷残基，可以缓解核积聚。这表明 3' 段腺苷可以促进输出，与裂解和聚腺苷酸化无关。我们进一步表明，合成的含有 A 尾的 RNA 在 3' 末端形成突变株中不受影响，其中正常切割和多聚腺苷酸化的 RNA 在细胞核内积累。我们的结果支持一个模型，其中多聚腺苷酸尾有助于有效的 mRNA 远离基因，很可能是通过酵母多聚腺苷酸尾结合蛋白 Pab1p 的作用。
To investigate the role of 3' end formation in yeast mRNA export, we replaced the mRNA cleavage and polyadenylation signal with a self-cleaving hammerhead ribozyme element. The resulting RNA is unadenylated and accumulates near its site of synthesis. Nonetheless, a significant fraction of this RNA reaches the cytoplasm. Nuclear accumulation was relieved by insertion of a stretch of DNA-encoded adenosine residues immediately upstream of the ribozyme element (a synthetic A tail). This indicates that a 3' stretch of adenosines can promote export, independently of cleavage and polyadenylation. We further show that a synthetic A tail-containing RNA is unaffected in 3' end formation mutant strains, in which a normally cleaved and polyadenylated RNA accumulates within nuclei. Our results support a model in which a polyA tail contributes to efficient mRNA progression away from the gene, most likely through the action of the yeast polyA-tail binding protein Pab1p.