O 6-Alkylguanine-DNA Alkyltransferase Activity in Normal Human Tissues and Cells
O 6-Alkylguanine-DNA Alkyltransferase Activity in Normal Human Tissues and Cells
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正常人体组织和细胞中的 O 6-烷基鸟嘌呤-DNA 烷基转移酶活性
DOI：
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
C. Harris
中科院分区：
文献类型：
--
作者：
Roland C. Graf strÃ;A. Pegg;B. Trump;C. Harris
正常成人组织和培养的支气管上皮细胞和成纤维细胞通过催化来自DMA的前诱变烷基化损伤O 6-甲基鸟嘌呤的修复而在体外表现出O 6-烷基鸟嘌呤-DNA烷基转移酶活性。肝脏、外周肺和结肠提取物的提取物的修复量与提取物蛋白质的量成比例。O 6甲基鸟嘌呤的修复导致了DMA中鸟嘌呤的化学计量再生和蛋白质中S-甲基半胱氨酸的化学计量形成。烷基转移酶活性在所测试的不同人体组织中以肝>结肠>食管>外周肺>脑的降序变化。肺组织提取物、培养的人支气管上皮细胞和成纤维细胞具有相似的烷基转移酶活性。各种人体组织的烷基转移酶活性比相应的大鼠组织高20 - 10倍。而在10个或10个以上的人肺和结肠标本中，活性的个体间变异为4 - 5倍，而在近交系大鼠中，个体间变异小于20%。目前的研究结果表明，不同的人体组织和细胞有几倍的能力，以修复O 6-甲基鸟嘌呤的DNA比大鼠组织和修复过程中发生的机制类似于以前在其他哺乳动物细胞和大肠杆菌。
Normal adult human tissues and cultured bronchial epithelial cells and fibroblasts exhibit 06-alkylguanine-DNA alkyltransfer ase activity in vitro by catalyzing the repair of the promutagenic alkylation lesion O6-methylguanine from DMA. The amount re paired by extracts of liver, peripheral lung, and colon extracts was proportional to the amount of extract protein. Repair of O6methylguanine led to stoichiometric regeneration of guanine in the DMA and stoichiometric formation of S-methylcysteine in protein. Alkyltransferase activity varies in the different human tissues tested in the decreasing order of liver > colon > esoph agus > peripheral lung > brain. Extracts of lung tissues, cultured human bronchial epithelial cells, and fibroblasts had similar al kyltransf erase activities. Various human tissues exhibit 2to 10fold higher alkyltransferase activity than corresponding rat tis sues. Whereas the interindividual variation of the activity was 4to 5-fold in ten or more human lung and colon specimens, the interindividual variation in the inbred rat was less than 20%. The present results show that different human tissues and cells have a several-fold higher capacity to repair O6-methylguanine in DNA than do rat tissues and that the repair process occurs via a mechanism similar to that shown previously in other mammalian cells and Escherichia coli.
通过人肝脏组分从 DNA 中去除 O6-甲基鸟嘌呤。
DOI：
10.1073/pnas.79.17.5162
发表时间：
1982
影响因子：
11.1
作者：
Pegg,AE;Roberfroid,M;vonBahr,C;Foote,RS;Mitra,S;Bresil,H;Likhachev,A;Montesano,R
通讯作者：
Montesano,R
大鼠肝脏 O6-甲基鸟嘌呤-DNA 转甲基酶的纯化和性质。
DOI：
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发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Pegg,AE;Wiest,L;Foote,RS;Mitra,S;Perry,W
通讯作者：
Perry,W
用 N-甲基-N-硝基-N-亚硝基胍处理的哺乳动物细胞中缺乏 O6-甲基鸟嘌呤-DNA 甲基转移酶的诱导。
影响因子：
4.7
作者：
Foote,RS;Mitra,S
通讯作者：
Mitra,S