Determination of total body water by deuterium isotope dilution measurements using chemical ionization mass spectrometry.
Determination of total body water by deuterium isotope dilution measurements using chemical ionization mass spectrometry.
复制标题
使用化学电离质谱法通过氘同位素稀释测量测定体内总水量。
DOI：
10.1002/bms.1200101104
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
F. H. Field
中科院分区：
文献类型：
--
作者：
F. Bencsath;H. Bradlow;F. H. Field
Two chemical ionization mass spectrometric methods were developed for direct determination of deuterium in water in the range of 0.0-0.6% 2H2O. One of them utilizes the batch inlet system, methane as the reagent gas, and the peak matching device of a magnetic sector mass spectrometer. The second one utilizes the directly-coupled gas chromatograph of a quadrupole mass spectrometer and computer control for ion selection and data processing. In this method the water itself serves as the reagent gas. The deuterium concentration is calculated from the ratio of ion intensities at m/z 20 (2HH2O+) and m/z 19 (H3O+). We have used these methods to determine total body water in 350 human subjects, which entailed making 900 measurements over a period of four years. Comparisons were made in 200 subjects of our results with those obtained by the creatinine method. No significant differences were found.
