Genetic diversity and genetic structure of consecutive breeding generations of golden mandarin fish (Siniperca scherzeri Steindachner) using microsatellite markers.
Genetic diversity and genetic structure of consecutive breeding generations of golden mandarin fish (Siniperca scherzeri Steindachner) using microsatellite markers.
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DOI：
10.4238/2015.september.25.1
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发表时间：
2015-09
期刊：
影响因子：
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通讯作者：
Luo Xn;Min Yang;Xu-Fang Liang;K. Jin;L. Lv;C. Tian;Yongchao Yuan;Jian Sun
中科院分区：
文献类型：
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作者：
Luo Xn;Min Yang;Xu-Fang Liang;K. Jin;L. Lv;C. Tian;Yongchao Yuan;Jian Sun
本研究利用12个多态性微卫星标记，对5个黄颡鱼召唤选育群体的遗传多样性和遗传结构进行了分析。等位基因总数、平均杂合度和平均多态信息含量表明，这些育种群体的遗传多样性呈下降趋势。从F_1代到后代，群体间的成对固定指数FST值和Da值逐渐增大（FST值在0.0221-0.1408之间，Da值在0.0608-0.1951之间）。分子变异分析表明，大部分遗传变异来自居群内个体（约占92.05%），居群间变异仅占7.95%。SC 75 -220和SC 101 -222 bp基因座的等位基因频率在5个世代中呈规律性变化。这两个位点的频率逐渐增加，并呈富集趋势，表明这两个位点与育种性状之间可能存在遗传相关。本研究表明，微卫星标记可以有效地用于评价金鳜鱼的遗传变异。
In this study, 12 polymorphic microsatellites were inves-tigated to determine the genetic diversity and structure of 5 consecu-tive selected populations of golden mandarin fish (Siniperca scherzeri Steindachner). The total numbers of alleles, average heterozyosity, and average polymorphism information content showed that the genetic diversity of these breeding populations was decreasing. Additionally, pairwise fixation index FST values among populations and Da values in-creased from F1 generation to subsequent generations (FST values from 0.0221-0.1408; Da values from 0.0608-0.1951). Analysis of molecular variance indicated that most genetic variations arise from individuals within populations (about 92.05%), while variation among populations accounted for only 7.95%. The allele frequency of the loci SC75-220 and SC101-222 bp changed regularly in the 5 breeding generations. Their frequencies were gradually increased and showed an enrichment trend, indicating that there may be genetic correlations between these 2 loci and breeding traits. Our study indicated that microsatellite markers are effective for assessing the genetic variability in the golden mandarin fish breeding program.