Expression in bladder transitional cell carcinoma by real-time quantitative reverse transcription polymerase chain reaction array of 65 genes at the tumor suppressor locus 9q34.1-2: Identification of 5 candidates tumor suppressor genes
Expression in bladder transitional cell carcinoma by real-time quantitative reverse transcription polymerase chain reaction array of 65 genes at the tumor suppressor locus 9q34.1-2: Identification of 5 candidates tumor suppressor genes
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DOI：
10.1002/ijc.20283
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发表时间：
2004-09-10
影响因子：
6.4
通讯作者：
Cussenot, O
中科院分区：
文献类型：
--
作者：
Amira, N;Cancel-Tassin, G;Cussenot, O
在膀胱移行细胞癌中，9q34.1-2的缺失是常见的。高缺失定位研究划定了标记D9 S61和D9 S66之间的临界区间，这是非常容易包含肿瘤抑制基因。通过实时定量RT-PCR分析位于该区域的65个基因的表达水平，比较肿瘤和正常尿路上皮。5个基因表现出显著降低的表达水平：C9 orf 9、KIAA 0625、ABL 1、LAMC 3和KIAA 1857-netrin-G2，其表现出最显著的下调（p = 0.0007）。KIAA 1857-netrin-G2属于netrins，可能是膀胱癌中的肿瘤抑制基因，因为netrin 1受体DCC与肿瘤发生有关。(C)2004 Wiley-Liss，Inc.
Frequent deletions on 9q34.1-2 were reported in bladder transitional cell carcinoma. High deletion mapping studies delimited a critical interval between markers D9S61 and D9S66, which is highly susceptible to contain a tumor suppressor gene. Expression level of the 65 genes localized in this region was analyzed by real-time quantitative RT-PCR, comparing tumor to normal urothelium. Five genes exhibited a significantly reduced expression level: C9orf9, KIAA0625, ABL1, LAMC3 and KIAA1857-netrin-G2, which exhibited the most significant downregulation (p = 0.0007). KIAA1857-netrin-G2 belongs to the netrins and might then be a tumor suppressor gene in bladder cancer, as netrin1 receptor DCC has been implicated in tumorigenesis. (C) 2004 Wiley-Liss, Inc.