The role of cellulose and O-antigen capsule in the colonization of plants by Salmonella enterica
The role of cellulose and O-antigen capsule in the colonization of plants by Salmonella enterica
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DOI：
10.1094/mpmi-20-9-1083
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发表时间：
2007-09-01
影响因子：
3.5
通讯作者：
Charkowski, Amy O.
中科院分区：
文献类型：
--
作者：
Barak, Jeri D.;Jahn, Courtney E.;Charkowski, Amy O.
许多沙门氏菌病的暴发都与蔬菜有关，特别是发芽的种子。薄聚集性菌毛(TAFI)是细胞外基质中负责多细胞行为的成分，对植物的肠道沙门氏菌附着和定植具有重要作用。在这里，我们证明了组成细胞外基质、纤维素和O-抗原胶囊的其他表面聚合物也在植物的定植中发挥了作用。细菌纤维素合成(BCSA)和O-抗原壳组装和易位(YIHO)的突变降低了附着和定植紫花苜蓿芽的能力。可食子酸突变体在植物附着或定植过程中不受影响。TAFI、纤维素合成和O-抗原被膜都参与了植物的附着和定植，这些都受到AGFD的调控，表明AGFD是沙门氏菌宿主外生存的关键调节因子。纤维素生物合成调节剂Adra突变体在附着或定植植物的能力上没有受到影响；然而，启动子探针分析显示附着在紫花苜蓿芽上的细胞表达。此外，定量逆转录聚合酶链式反应显示AGFD和Adra在浮游细胞和植物附着细胞中的表达存在差异。此外，不同突变体之间的生物膜形成和对植物的附着之间没有相关性。在动物宿主之外，肠杆菌似乎依赖大量的粘附素来维持对植物的依赖，这些植物可以充当媒介，使公共卫生问题永久化。
Numerous salmonellosis outbreaks have been associated with vegetables, in particular sprouted seed. Thin aggregative fimbriae (Tafi), a component of the extracellular matrix responsible for multicellular behavior, are important for Salmonella enterica attachment and colonization of plants. Here, we demonstrate that the other surface polymers composing the extracellular matrix, cellulose, and O-antigen capsule also play a role in colonization of plants. Mutations in bacterial cellulose synthesis (bcsA) and O-antigen capsule assembly and translocation (yihO) reduced the ability to attach to and colonize alfalfa sprouts. A colanic acid mutant was unaffected in plant attachment or colonization. Tafi, cellulose synthesis, and O-antigen capsule, all of which contribute to attachment and colonization of plants, are regulated by AgfD, suggesting that AgfD is a key regulator for survival outside of hosts of Salmonella spp. The cellulose biosynthesis regulator adrA mutant was not affected in the ability to attach to or colonize plants; however, promoter probe assays revealed expression by cells attached to alfalfa sprouts. Furthermore, quantitative reverse-transcriptase polymerase chain reaction revealed differential expression of agfD and adrA between planktonic and plant-attached cells. In addition, there was no correlation among mutants between biofilm formation in culture and attachment to plants. Outside of animal hosts, S. enterica appears to rely on an arsenal of adhesins to persist on plants, which can act as vectors and perpetuate public health concerns.