DNA bending is a determinant of binding specificity for a Drosophila zinc finger protein.
DNA bending is a determinant of binding specificity for a Drosophila zinc finger protein.
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DNA 弯曲是果蝇锌指蛋白结合特异性的决定因素。
DOI：
10.1101/gad.4.9.1505
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发表时间：
1990
影响因子：
10.5
通讯作者：
Corces,VG
中科院分区：
文献类型：
--
作者：
Spana,C;Corces,VG
毛翼[su（Hw）]基因座的抑制基因编码一种含锌指的蛋白质，该蛋白质与果蝇吉普赛元件的特定序列结合，介导该逆转录转座子的诱变效应。我们进行了详细的分析，苏（HW）-吉普赛人的相互作用，使用各种生化测定。DNA酶I足迹法在gypsy的编码链中界定了一个37 bp的区域，该区域通过su（Hw）蛋白的结合而免受消化。通过甲基化保护分析和使用羟基自由基的缺失核苷实验来确定参与相互作用的特定DNA接触。从这些实验的结果表明，su（Hw）蛋白结合的序列同源的八聚体基序，这是承认的同源框包含蛋白在哺乳动物生物体。此外，在不存在蛋白质的情况下，存在于结合位点两侧的两个DNA弯曲有利于这种相互作用的强度。
The suppressor of Hairy-wing [su(Hw)] locus encodes a zinc finger-containing protein that binds to specific sequences of the Drosophila gypsy element, mediating the mutagenic effects of this retrotransposon. We carried out a detailed analysis of the su(Hw)-gypsy interaction using various biochemical assays. DNase I footprinting delimits a 37-bp region in the coding strand of gypsy that is protected from digestion by the binding of the su(Hw) protein. Specific DNA contacts involved in the interaction were determined by methylation protection analysis and by missing nucleoside experiments using hydroxyl radical. Results from these experiments indicate that the su(Hw) protein binds to sequences homologous to the octamer motif, which is recognized by homeo box-containing proteins in mammalian organisms. Furthermore, two DNA bends present on both sides of the binding site in the absence of protein favor the strength of this interaction.