Reactions of aggregated mercaptoethanol treated gamma globulin with rheumatoid factor--precipitin and complement fixation studies.
Reactions of aggregated mercaptoethanol treated gamma globulin with rheumatoid factor--precipitin and complement fixation studies.
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聚集的巯基乙醇处理的丙种球蛋白与类风湿因子的反应——沉淀素和补体固定研究。
DOI：
10.1002/art.1780110402
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发表时间：
1968
影响因子：
--
通讯作者：
Peter H. Schur
中科院分区：
文献类型：
--
作者：
N. J. Zvaifler;Peter H. Schur
人γ球蛋白在聚集前的还原和烷基化导致补体结合能力丧失，并保留与类风湿因子反应的能力。向含有高效价类风湿因子的血清中加入聚集的还原型丙种球蛋白，产生典型的钟形沉淀曲线，在抗原过量区域有一个等当点和少量沉淀。由于聚集的还原型丙种球蛋白本身几乎不固定补体，因此可以确定它与类风湿因子的反应是否固定补体。在适当的条件下类风湿因子可以固定补体。当聚集时，将还原的γ球蛋白加入到含有不同升类风湿因子的热灭活血清中，未观察到豚鼠或人补体的固定。但是类风湿因子和丙种球蛋白的预制复合物在37 ℃时可以固定人的补体.皮内注射40毫克N的聚集，还原γ球蛋白到对照组或类风湿性关节炎患者产生没有立即或延迟的皮肤反应。
Reduction and alkylation of human gamma globulin prior to aggregation results in a loss of complement-fixing ability, and retention of the capacity to react with rheumatoid factor. The addition of aggregated, reduced gamma globulin to serum containing high tilers of rheumatoid factor produces a typical bell-shaped precipitin curve with an equivalence point and little precipitate in the region of antigen excess. Since aggregated, reduced gamma globulin fixes little complement by itself, it was possible to determine whether its reaction with rheumatoid factor fixes complement. Under appropriate conditions rheumatoid factor can fix complement. When aggregated, reduced gamma globulin was added to heat-inactivated sera with varying liters of rheumatoid factor, no fixation of guinea pig or human complement was observed. But preformed complexes of rheumatoid factor and gamma globulin will fix human complement at 37 C. Intradermal injection of 40 mg N of aggregated, reduced gamma globulin into control subjects or patients with rheumatoid arthritis produced no immediate or delayed skin reactions.