Influence of culture modes on the microbial production of hyaluronic acid by Streptococcus zooepidemicus
Influence of culture modes on the microbial production of hyaluronic acid by Streptococcus zooepidemicus
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DOI：
10.1007/s12257-007-0193-7
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发表时间：
2008-08
影响因子：
3.2
通讯作者：
Long Liu;G. Du;Jian Chen;Miao Wang;Jun Sun
中科院分区：
文献类型：
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作者：
Long Liu;G. Du;Jian Chen;Miao Wang;Jun Sun
The principal objective of this study was to assess the effects of culture modes including batch culture, pulse fed-batch culture, constant feeding rate fed-batch culture, and exponential fed-batch culture on the production of hyaluronic acid (HA) byStreptococcus zooepidemicus. Batch cultures had the highest levels of HA productivity, whereas fed-batch cultures were more favorable with regard to cell growth, and exponential fed-batch cultures evidenced the highest cell concentrations. A two-step culture model was proposed to enhance HA production: an exponential fed-batch culture was conducted prior to 8 h and then sucrose supplementation was applied for 8 h to start the batch fermentation ofS. zooepidemicus. HA production and productivity were increased by 36 and 37% in the proposed two-step culture process as compared with that observed in the batch culture, respectively. The proposed two-step culture model can be applied in the production of secondary metabolites, and particularly of the exopolysaccharides.
