Inter-study reproducibility of arterial spin labelling magnetic resonance imaging for measurement of renal perfusion in healthy volunteers at 3 Tesla.
Inter-study reproducibility of arterial spin labelling magnetic resonance imaging for measurement of renal perfusion in healthy volunteers at 3 Tesla.
复制标题
DOI：
10.1186/1471-2369-15-23
复制
发表时间：
2014-01-31
期刊：
影响因子：
2.3
通讯作者：
Mark PB
中科院分区：
文献类型：
--
作者：
Gillis KA;McComb C;Foster JE;Taylor AH;Patel RK;Morris ST;Jardine AG;Schneider MP;Roditi GH;Delles C;Mark PB
肾灌注的测量是测量肾功能的重要组成部分。动脉自旋标记磁共振成像 (ASL MRI) 是一种使用磁化血液作为内源性对比来测量肾灌注的非侵入性方法。我们研究了正常志愿者中 ASL MRI 的再现性。使用 3.0 Tesla MRI 扫描仪对健康志愿者进行 2 次 ASL MRI 扫描，该扫描仪配有流量敏感交替反转恢复 (FAIR) 灌注准备和稳态自由进动 (True-FISP) 脉冲序列。根据扫描图像测量肾脏体积。在 MRI 扫描之前测量常规血清和尿液生化。招募了 12 名志愿者，产生 24 个肾脏，参与者平均年龄为 44.1 ± 14.6 岁，血压为 136/82 mmHg，慢性肾脏病流行病学公式估计肾小球滤过率 (CKD EPI eGFR) 为 98.3 ± 15.1 ml/min/1.73 m2。使用椭球公式和体素计数方法测量的平均肾脏体积分别为 123.5 ± 25.5 cm3 和 156.7 ± 28.9 cm3。平均肾脏灌注为 229 ± 41 ml/min/100 g，平均皮质灌注为 327 ± 63 ml/min/100 g，ASL MRI 之间没有显着差异。根据扫描期间测量的肾脏体积计算出的平均绝对肾脏灌注为 373 ± 71 ml/min。 Bland Altman 图是根据 ASL MRI 1 和 2 进行的皮质和全肾灌注测量构建的。这些显示测量之间具有良好的一致性，根据观察到的差异绘制平均值的随机分布。皮质灌注的组内相关性为 0.85，而受试者内方差系数为 9.2%。全肾灌注的组内相关性为 0.86，而受试者内方差系数为 7.1%。 3.0 特斯拉的 ASL MRI 提供了一种测量健康受试者肾灌注的可重复方法，无需施用外源化合物。我们使用 ASL MRI 在一组健康志愿者中建立了肾灌注的正常值。
Measurement of renal perfusion is a crucial part of measuring kidney function. Arterial spin labelling magnetic resonance imaging (ASL MRI) is a non-invasive method of measuring renal perfusion using magnetised blood as endogenous contrast. We studied the reproducibility of ASL MRI in normal volunteers. ASL MRI was performed in healthy volunteers on 2 occasions using a 3.0 Tesla MRI scanner with flow-sensitive alternating inversion recovery (FAIR) perfusion preparation with a steady state free precession (True-FISP) pulse sequence. Kidney volume was measured from the scanned images. Routine serum and urine biochemistry were measured prior to MRI scanning. 12 volunteers were recruited yielding 24 kidneys, with a mean participant age of 44.1 ± 14.6 years, blood pressure of 136/82 mmHg and chronic kidney disease epidemiology formula estimated glomerular filtration rate (CKD EPI eGFR) of 98.3 ± 15.1 ml/min/1.73 m2. Mean kidney volumes measured using the ellipsoid formula and voxel count method were 123.5 ± 25.5 cm3, and 156.7 ± 28.9 cm3 respectively. Mean kidney perfusion was 229 ± 41 ml/min/100 g and mean cortical perfusion was 327 ± 63 ml/min/100 g, with no significant differences between ASL MRIs. Mean absolute kidney perfusion calculated from kidney volume measured during the scan was 373 ± 71 ml/min. Bland Altman plots were constructed of the cortical and whole kidney perfusion measurements made at ASL MRIs 1 and 2. These showed good agreement between measurements, with a random distribution of means plotted against differences observed. The intra class correlation for cortical perfusion was 0.85, whilst the within subject coefficient of variance was 9.2%. The intra class correlation for whole kidney perfusion was 0.86, whilst the within subject coefficient of variance was 7.1%. ASL MRI at 3.0 Tesla provides a repeatable method of measuring renal perfusion in healthy subjects without the need for administration of exogenous compounds. We have established normal values for renal perfusion using ASL MRI in a cohort of healthy volunteers.