Transplantation of brain cells assembled around a programmable synthetic microenvironment
Transplantation of brain cells assembled around a programmable synthetic microenvironment
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DOI：
10.1038/nbt1001-934
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发表时间：
2001-10-01
影响因子：
46.9
通讯作者：
Saltzman, WM
中科院分区：
文献类型：
--
作者：
Mahoney, MJ;Saltzman, WM
细胞疗法是治疗造血系统疾病、神经退行性疾病、糖尿病和创伤引起的组织损失的有前景的方法。细胞治疗的一些主要障碍已经部分解决，包括细胞群的鉴定，体外细胞增殖和免疫抑制策略。一个未解决的问题是重演的独特组合的基质，生长因子和细胞粘附的线索，区分每个干细胞微环境，这是至关重要的祖细胞分化和组织发生的控制。在这里，我们描述了一种方法，其中细胞，合成基质元素，和控制释放技术组装和编程，移植前，模仿化学和物理微环境的发展组织。我们证明了这种方法在动物中使用的移植系统，允许控制胎儿脑细胞的存活和分化的新组织含有细胞和神经生长因子（NGF）释放的合成颗粒的预组装。
Cell therapy is a promising method for treatment of hematopoietic disorders, neurodegenerative diseases, diabetes, and tissue loss due to trauma. Some of the major barriers to cell therapy have been partially addressed, including identification of cell populations, in vitro cell proliferation, and strategies for immunosuppression. An unsolved problem is recapitulation of the unique combinations of matrix, growth factor, and cell adhesion cues that distinguish each stem cell microenvironment, and that are critically important for control of progenitor cell differentiation and histogenesis. Here we describe an approach in which cells, synthetic matrix elements, and controlled-release technology are assembled and programmed, before transplantation, to mimic the chemical and physical microenvironment of developing tissue. We demonstrate this approach in animals using a transplantation system that allows control of fetal brain cell survival and differentiation by preassembly of neo-tissues containing cells and nerve growth factor (NGF)-releasing synthetic particles.