King Grass: A very promising material for the production of second generation ethanol in tropical countries
King Grass: A very promising material for the production of second generation ethanol in tropical countries
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DOI：
10.1016/j.biombioe.2016.10.008
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发表时间：
2016-12-01
影响因子：
6
通讯作者：
Rios, Luis
中科院分区：
文献类型：
--
作者：
Cardona, Eliana;Rios, Jorge;Rios, Luis
王草是一种生长迅速，产量高，杂交草，生长在各种土壤条件下，不与粮食作物竞争。王草是多年生植物，每年可以收获几次。因此，它在热带国家生产乙醇的潜力很大。预处理包括碱脱木质素（66.6-133 ℃，30-180 min，固液比1：13.3-1：20 wt，NaOH浓度0.7- 2%wt），稀酸水解（2%wt硫酸，固液比为1.0 g生物质/mL溶液，在90 ℃搅拌90 min），蒸汽爆破（在180 ℃ 5 min，即，强度3.05）、碱性过氧化物（2%vol.H2O2，液固比为20：1重量，pH 11.5，在35 ℃下搅拌3小时）和氨水浸泡（15%wt氨溶液，每克材料8 mL氨溶液，在60 ℃下6小时）。用NaOH的碱性预处理导致还原糖的最高浓度，即，58.6 g/L，并达到最高乙醇浓度，即，17.9微克/升对碱预处理条件（温度、固液比、NaOH浓度和停留时间）对王草乙醇生产的影响进行了更详细的研究。在120 ° C下用2%wt NaOH预处理1小时并且固液比为1：20的条件下，获得了迄今为止报道的最高乙醇产率，即，27.7 g/L（165.9 mg乙醇/g干生物质，理论产率的92%）。此外，这种预处理允许去除王草中存在的大部分木质素，即，94%，并且允许固体中纤维素级分的高回收率（93-99%）。在哥伦比亚王草的典型生长条件下，该最大乙醇产率对应于12，616 L/ha/年，其高于从甘蔗渣获得的乙醇产率（4602 L/ha/年）。(C)2016爱思唯尔有限公司版权所有。
King Grass is a fast growing, high yield, hybrid grass that grows in a variety of soil conditions and does not compete with food crops. King Grass is perennial and can be harvested several times per year. Therefore it has a high potential for ethanol production in tropical countries. The pretreatments alkaline delignification (66.6-133 degrees C, 30-180 min, solid to liquid ratio 1:13.3-1:20 wt and NaOH concentration 0.7-2% wt), diluted acid hydrolysis (2% wt sulfuric acid, solid to liquid ratio of 1.0 g biomass/mL solution, stirring at 90 degrees C for 90 min), steam explosion (at 180 degrees C for 5 min, i.e., severity 3.05), alkaline peroxide (2% vol. H2O2, liquid to solid ratio of 20:1 wt, pH 11.5, stirring at 35 degrees C for 3 h) and aqueous ammonia soaking (15% wt ammonia solution, 8 mL of ammonia solution per gram of material, at 60 degrees C for 6 h) were studied. The alkaline pretreatment with NaOH led to the highest concentration of reducing sugars, i.e., 58.6 g/L, and to the highest ethanol concentration, i.e., 17.9 g/L. A more detailed study of the effect of the alkaline pretreatment conditions (temperature, solid to liquid ratio, NaOH concentration and residence time) on the production of ethanol from King Grass was carried out. Under pretreatment conditions of 120 degrees C for 1 h with 2% wt NaOH and a solid to liquid ratio of 1:20 the highest ethanol yield reported so far was obtained, i.e., 27.7 g/L (165.9 mg ethanol/g dry biomass, 92% of theoretical yield). Furthermore, this pretreatment allowed the removal of most of lignin present in King Grass, i.e., 94%, and allowed a high recovery of the cellulosic fraction in the solid (93-99%). Under typical growing conditions of King Grass in Colombia this maximum ethanol yield corresponds to 12,616 L/ha/year which is higher than that obtained from sugar cane bagasse (4602 L/ha/year). (C) 2016 Elsevier Ltd. All rights reserved.