The effects of the nanotopography of biomaterial surfaces on pseudomonas fluorescens cell adhesion
The effects of the nanotopography of biomaterial surfaces on pseudomonas fluorescens cell adhesion
复制标题
生物材料表面纳米形貌对荧光假单胞菌细胞粘附的影响
DOI：
10.1016/j.bej.2004.07.011
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发表时间：
2004-12-01
影响因子：
3.9
通讯作者：
Chen, YT
中科院分区：
文献类型：
--
作者：
Li, XQ;Liu, TQ;Chen, YT
采用层层自组装的方法构建了纳米Al_2O_3和纳米SiO_2颗粒修饰表面。用原子力显微镜对纳米粒子表面和对照表面进行了表面形貌分析。荧光假单胞菌(PF)细胞悬液在动态和静态条件下与对照组和修饰表面的粘附数通过显微镜检测来评价。结果表明，PF细胞在两种表面上的粘附数均随暴露时间的延长而增加，且在修饰后的表面上的粘附数明显高于对照组。同时，倒置显微镜观察了纳米粒子修饰表面的PF细胞形态，结果表明，大多数修饰表面的PF细胞以团簇的形式存在，在细胞黏附的后期占细胞总黏附数的绝大部分；而在对照组，PF细胞几乎没有融合。结果表明，纳米粒子修饰的表面可以增强细菌与材料之间的相互作用。(C)2004爱思唯尔B.V.保留所有权利。
The nano-Al2O3 and nanO-SiO2 particle-modified surfaces were built up by Layer-by-Layer self-assembled method. The properties of these nanoparticle-modified surfaces and the controls were investigated with atomic force microscope for topography analysis. Pseudomonas fluorescens (PF) cell adhesion was evaluated by microscopic determination of the numbers of cells that adhered to the controls and modified surfaces exposed to PF cell suspension under dynamic and static conditions. The results show that the adhesion numbers of PF cells on both surfaces readily increase with the exposition time, and the number on the modified surfaces is much more than that on the controls. Meanwhile, the PF cell morphologies on these nanoparticle-modified surfaces studied by inverted microscope show that the majority of PF cells adhering to the modified surfaces are in presence of clusters, which contributes overwhelming amount to the total adhesion numbers during the later time of cell adhesion; while on the controls, few PF cells are confluent. The results suggest that nanoparticle-modified surfaces may enhance the interactions between bacteria and materials. (C) 2004 Elsevier B.V. All rights reserved.