Cloning of the gene coding for staphylococcal exfoliative toxin A and its expression in Escherichia coli
Cloning of the gene coding for staphylococcal exfoliative toxin A and its expression in Escherichia coli
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葡萄球菌剥脱性毒素A基因的克隆及其在大肠杆菌中的表达
DOI：
10.1111/j.1574-6968.1987.tb02300.x
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发表时间：
1987
影响因子：
2.1
通讯作者：
I. Kondo
中科院分区：
文献类型：
--
作者：
S. Sakurai;Hitoshi Suzuki;I. Kondo
从基因组DNA中克隆出A型葡萄球菌剥脱毒素（ETA）基因，并在大肠埃希菌C600中表达。重组质粒pETA-J3从产生eta的转化中恢复，eta基因位于1.7 kb的Alu I片段内。大肠杆菌转化产生的ETA （cETA）在理化、血清学性质和剥脱毒性方面与金黄色葡萄球菌（sETA）产生的ETA没有区别。cETA未保留在质周空间；超过97%的毒素存在于细胞外。
The gene for the staphylococcal exfoliative toxin of serotype A (ETA) was cloned from the genomic DNA into pUC9 and expressed in Escherichia coli C600. Recombinant plasmid pETA-J3 was recovered from an ETA-producing transformant and the eta gene was shown to be located within a 1.7-kb Alu I fragment. ETA produced by the E. coli transformant, cETA, was indistinguishable from that produced by Staphylococcus aureus, sETA, in its physicochemical and serological properties and exfoliative toxicity. cETA was not retained in the periplasmic space; and more than 97% of the toxin was extracellular.