Effect of colostrum composition on passive calf immunity in primiparous and multiparous dairy cows
Effect of colostrum composition on passive calf immunity in primiparous and multiparous dairy cows
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DOI：
10.17221/40/2017-vetmed
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发表时间：
2018-01-01
影响因子：
0.7
通讯作者：
Guzelbektes, H.
中科院分区：
文献类型：
--
作者：
Aydogdu, U.;Guzelbektes, H.
本研究的目的是确定初乳质量和组成对经产和初产奶牛犊牛被动免疫的影响。本研究使用了24头经产奶牛和24头经产奶牛。第一组为经产奶牛产下的小牛，第二组为经产奶牛产下的小牛。出生后，立即从奶牛中采集初乳样品。在第一次初乳摄入前和第一次初乳摄入后第2、7、14和28天从小牛中采集静脉血样。分析血液和初乳样本的生化参数、免疫球蛋白和矿物质水平。分别采用Gerber法和凯氏定氮法测定初乳中的脂肪和粗蛋白水平。经产牛初乳中免疫球蛋白含量显著高于初产牛（P < 0.05），而脂肪率、LDH活性、Ca、Mg、P、K含量显著低于初产牛（P < 0.05）。初乳免疫球蛋白、γ-谷氨酰转移酶、粗蛋白和总蛋白之间呈正相关。所有犊牛的血清免疫球蛋白、总蛋白、球蛋白和γ-谷氨酰转移酶活性在饲喂初乳后均升高。但第二组犊牛的血清免疫球蛋白、总蛋白、球蛋白和γ-谷氨酰转移酶水平均高于第一组犊牛。血清免疫球蛋白、γ-谷氨酰转移酶、球蛋白和总蛋白之间呈正相关。铁浓度在所有小牛下降的过程中的14天，并在第二组小牛相比，第一组。本研究结果表明，经产牛初乳品质优于非经产牛。初乳粗蛋白、总蛋白、γ-谷氨酰转移酶沿着和初乳免疫球蛋白可用于初乳质量的检测。血清免疫球蛋白、总蛋白、球蛋白和γ-谷氨酰转移酶活性可作为判断犊牛被动转移状态的指标。
The aim of this study was to determine the effect of colostrum quality and composition on passive calf immunity in primiparous and multiparous dairy cows. Twenty-four primiparous and 24 multiparous dairy cows were used in this study. Calves born from primiparous dairy cows comprised the first group and calves born from multiparous dairy cows constituted the second group. After birth, colostrum samples were immediately taken from dairy cows. Venous blood samples were collected from the calves before the first colostrum intake and on the 2nd, 7th, 14th and 28th days after the first colostrum intake. Blood and colostrum samples were analysed for biochemical parameters, immunoglobulin and mineral levels. Fat and crude protein levels in colostrum were determined using the Gerber and Kjeldahl methods, respectively. Immunoglobulin levels in the colostrum of multiparous cows were significantly (P < 0.05) higher compared to primiparous cows while fat ratio, LDH activity, Ca, Mg, P and K levels were lower. There was a positive correlation among colostrum immunoglobulin, gamma-glutamyltransferase, crude protein and total protein. Serum immunoglobulin, total protein, globulin and gamma-glutamyltransferase activity in all calves were increased following the colostrum feeding. However, the serum immunoglobulin, total protein, globulin and gamma-glutamyltransferase levels in the second group of calves were higher than those of the first group of calves. There was a positive correlation among serum immunoglobulin, gamma-glutamyltransferase, globulin and total protein. Fe concentrations in all calves decreased over the course of 14 days and were lower in the second group of calves compared to the first group. In conclusion, the results of this study show that the colostrum quality of multiparous cows was better than that of primiparous cows. Colostrum crude protein, total protein, gamma-glutamyltransferase along with colostrum immunoglobulin might be used to determine colostrum quality. Serum immunoglobulin, total protein, globulin and gamma-glutamyltransferase activities could be used to determine the passive transfer status of calves.