Visualization of the Oncolytic Alphavirus M1 Life Cycle in Cancer Cells
Visualization of the Oncolytic Alphavirus M1 Life Cycle in Cancer Cells
复制标题
癌细胞中溶瘤甲病毒 M1 生命周期的可视化
DOI：
10.1007/s12250-020-00339-7
复制
发表时间：
2021-01-22
影响因子：
5.5
通讯作者：
Zhang, Qinfen
中科院分区：
文献类型：
--
作者：
Dan, Jia;Nie, Lin;Zhang, Qinfen
溶瘤甲病毒M1已显示出选择性靶向和杀死癌细胞，但M1病毒诱导的细胞病变形态和M1毒株在癌细胞中的生命周期仍不清楚。在这里，我们研究的M1病毒感染和复制的M1病毒敏感的HepG2肝癌细胞系的关键阶段，通过透射电子显微镜，特别是检查病毒进入，组装，成熟和释放。我们发现，M1病毒在感染过程中诱导癌细胞空泡化，并最终诱导细胞核边缘化，这是细胞凋亡的典型指标。具体来说，我们的研究结果表明，内质网参与核衣壳的组装。在感染的早期和晚期，形成了三种特殊的细胞病变空泡，它们似乎参与了病毒的复制、成熟和释放。这些结果揭示了M1病毒感染肿瘤细胞的过程，为研究M1病毒与宿主的相互作用提供了结构基础。
Oncolytic alphavirus M1 has been shown to selectively target and kill cancer cells, but cytopathic morphologies induced by M1 virus and the life cycle of the M1 strain in cancer cells remain unclear. Here, we study the key stages of M1 virus infection and replication in the M1 virus-sensitive HepG2 liver cancer cell line by transmission electron microscopy, specifically examining viral entry, assembly, maturation and release. We found that M1 virus induces vacuolization of cancer cells during infection and ultimately nuclear marginalization, a typical indicator of apoptosis. Specifically, our results suggest that the endoplasmic reticulum participates in the assembly of nucleocapsids. In the early and late stage of infection, three kinds of special cytopathic vacuoles are formed and appear to be involved in the replication, maturation and release of the virus. Taken together, our data displayed the process of M1 virus infection of tumor cells and provide the structural basis for the study of M1 virus-host interactions.