Modulation of Neuronal Activity by Glial Cells in the Retina
Modulation of Neuronal Activity by Glial Cells in the Retina
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DOI：
10.1523/jneurosci.18-11-04022.1998
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发表时间：
1998-06
期刊：
影响因子：
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通讯作者：
E. Newman;K. Zahs
中科院分区：
文献类型：
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作者：
E. Newman;K. Zahs
在大鼠眼杯标本中，通过监测细胞间胶质细胞Ca ~（2+）波对邻近神经元电活动的影响，研究了胶质细胞-神经元通讯。在星形胶质细胞和Müller细胞中的钙波由施加到视网膜表面的机械刺激启动。当且仅当这些Ca 2+波到达神经元时，神经节细胞层内神经元的光诱发尖峰活动发生变化。在53个神经元中的25个中观察到活性抑制（峰频率平均降低，28 ± 2%）。另外5个神经元出现兴奋（平均增加27 ± 5%）。较大幅度的钙波与神经元活动的更大调制。Thapsigargin，减少了神经胶质细胞Ca 2+增加的幅度，也减少了神经元调制的幅度。抑制性神经递质拮抗剂荷包牡丹碱和士的宁，以及谷氨酸拮抗剂6-硝基-7-氨磺酰基苯并[f]喹喔啉-2，3-二酮（NBQX）和d（−）-2-氨基-7-膦酰基庚酸（D-AP 7），减少了与胶质细胞钙波相关的神经元活动抑制，表明抑制是由胶质细胞释放谷氨酸刺激的抑制性中间神经元介导的。结果表明，神经胶质细胞能够调节视网膜内神经元的电活动，因此，可能直接参与中枢神经系统的信息处理。
Glial–neuronal communication was studied by monitoring the effect of intercellular glial Ca2+ waves on the electrical activity of neighboring neurons in the eyecup preparation of the rat. Calcium waves in astrocytes and Müller cells were initiated with a mechanical stimulus applied to the retinal surface. Changes in the light-evoked spike activity of neurons within the ganglion cell layer occurred when, and only when, these Ca2+ waves reached the neurons. Inhibition of activity was observed in 25 of 53 neurons (mean decrease in spike frequency, 28 ± 2%). Excitation occurred in another five neurons (mean increase, 27 ± 5%). Larger amplitude Ca2+ waves were associated with greater modulation of neuronal activity. Thapsigargin, which reduced the amplitude of the glial Ca2+ increases, also reduced the magnitude of neuronal modulation. Bicuculline and strychnine, inhibitory neurotransmitter antagonists, as well as 6-Nitro-7-sulphamoylbenzo[f]quinoxaline-2,3-dione (NBQX) andd(−)-2-amino-7-phosphonoheptanoic acid (D-AP7), glutamate antagonists, reduced the inhibition of neuronal activity associated with glial Ca2+ waves, suggesting that inhibition is mediated by inhibitory interneurons stimulated by glutamate release from glial cells. The results suggest that glial cells are capable of modulating the electrical activity of neurons within the retina and thus, may directly participate in information processing in the CNS.