Monitoring the Rhizopus oryzae lipase catalyzed hydrolysis of castor oil by ATR-FTIR spectroscopy
Monitoring the Rhizopus oryzae lipase catalyzed hydrolysis of castor oil by ATR-FTIR spectroscopy
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DOI：
10.1016/j.molcatb.2015.01.002
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发表时间：
2015-03-01
影响因子：
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通讯作者：
Afridi, Hassan Imran
中科院分区：
文献类型：
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作者：
Khaskheli, Abid Ali;Talpur, Farah N.;Afridi, Hassan Imran
建立了一种环境友好的衰减全反射傅里叶变换红外（ATR-FTIR）光谱法，用于监测水包油乳状液体系中根霉脂肪酶（ROL）催化蓖麻油水解反应。通过在蓖麻油中重量添加油酸（10-50%），使用偏最小二乘（PLS）模型构建校准曲线，以检测区域（1690-1730 cm（-1））内游离脂肪酸（FFA）的羰基吸收。PIS模型的相关系数（R-2）和校正均方根误差（RMSEC）分别为0.999和0.316。ROL被发现是一种有效的生物催化剂，从蓖麻油生产游离脂肪酸（FFA）。对影响水解率的因素进行了优化，确定了酶浓度为0.01%，pH为7，温度为37 ℃，油水比为1：4，反应时间为12 h。在所有条件下，ROL有效水解蓖麻油，脂肪酸产率高达90%。该方法在两种情况下都是相当环保的，即使用脂肪酶水解蓖麻油并通过FTIR光谱分析产物。(C)2015爱思唯尔B. V.保留所有权利。
A rapid and environmental friendly Attenuated total reflectance Fourier-transform infrared (ATR-FTIR) spectroscopic method was developed for monitoring the Rhizopus oryzae lipase (ROL) catalyzed hydrolysis of castor oil in oil-in-water emulsion system. A calibration curve was constructed using partial least square (PLS) model by gravimetric addition of oleic acid (10-50%) in castor oil to detect the carbonyl absorption of free fatty acids (FFA) in the region (1690-1730 cm(-1)). The correlation co-efficient (R-2) and root mean square error of calibration (RMSEC) by PIS model were found to be 0.999 and 0.316, respectively. ROL was found to be an efficient biocatalyst to produce free fatty acids (FFA) from castor oil. Factors affecting the rate of hydrolysis such as enzyme concentration (0.01%, w/v), pH (7), temperature (37 degrees C), oil-water ratio (1:4) and reaction time (12 h) were optimized. Under all set of conditions the ROL effectively hydrolyzed castor oil up to 90% yield of fatty acids. The methodology is fairly environmental friendly in both cases, i.e. using lipase for hydrolysis of castor oil and analyzing the product through FTIR spectroscopy. (C) 2015 Elsevier B.V. All rights reserved.