ZntR is a Zn(II)-responsive MerR-like transcriptional regulator of zntA in Escherichia coli
ZntR is a Zn(II)-responsive MerR-like transcriptional regulator of zntA in Escherichia coli
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DOI：
10.1046/j.1365-2958.1999.01229.x
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发表时间：
1999-02-01
影响因子：
3.6
通讯作者：
Morby, AP
中科院分区：
文献类型：
--
作者：
Brocklehurst, KR;Hobman, JL;Morby, AP
我们已经确定了启动子/操作子区的大肠杆菌的zntA基因，并表明，锌（II）是主要的诱导剂表达的Zn（II）/Cd（II）出口基因。启动子PzntA显示出与耐汞（mer）操纵子的启动子的序列相似性，包括含有反向重复序列的长间隔区。已通过基因产物的表达和插入失活/互补从基因组序列数据中鉴定了编码PzntA转录调节因子的基因，命名为zntR。ZntR产物是MerR家族的转录调节因子的成员，并且似乎充当超敏感的转录开关。已经构建了一个混合MerR/ZntR蛋白，并表明ZntR的C-末端区域识别Zn（II）。
We have identified the promoter/operator region of the zntA gene of Escherichia coli and shown that Zn(II) is the primary inducer of expression of this Zn(II)/Cd(II) export gene. The promoter PzntA shows sequence similarities to the promoters of mercury resistance (mer) operons, including a long spacer region containing an inverted repeat sequence. The gene encoding the transcriptional regulator of PzntA, designated zntR, has been identified from genome sequence data, by expression of the gene product and by insertional inactivation/complementation. The ZntR product is a member of the MerR family of transcriptional regulators and appears to act as a hypersensitive transcriptional switch. A hybrid MerR/ZntR protein has been constructed and indicates that the C-terminal region of ZntR recognizes Zn(II).