Protection of feruloylated oligosaccharides from corn bran against oxidative stress in PC 12 cells.
Protection of feruloylated oligosaccharides from corn bran against oxidative stress in PC 12 cells.
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DOI：
10.1021/jf404841c
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发表时间：
2014-01
影响因子：
6.1
通讯作者：
Sheng-wen Yao;Xiao-xiao Wen;Ru-qing Huang;Rong-Rong He-Rong;S. Ou;W. Shen;Caihuan Huang;Xichun Peng
中科院分区：
文献类型：
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作者：
Sheng-wen Yao;Xiao-xiao Wen;Ru-qing Huang;Rong-Rong He-Rong;S. Ou;W. Shen;Caihuan Huang;Xichun Peng
通过在草酸（0.6%）溶液中高压灭菌玉米麸制备阿魏酰化寡糖（FO），并研究其对嗜铬细胞瘤细胞（PC 12）细胞氧化应激的保护作用。 FO 样品包含阿魏酰化单戊糖和二戊糖与 4.88% 结合阿魏酸 (FA) 的混合物，以及分别占总糖 46.43%、40.46%、3.76% 和 8.68% 的木糖、阿拉伯糖、半乳糖和葡萄糖，通过将预处理的玉米麸在 0.6% 草酸中高压灭菌来制备。和 然后进一步分离。通过1,1-二苯基-2-三硝基苯肼自由基2,2-二苯基-1-(2,4,6-三硝基苯基)肼基(DPPH)清除和氧自由基吸收能力(ORAC)方法测试抗氧化活性。在 PC 12 神经元细胞培养模型中，氧化应激由 H2O2 诱导。结果表明，FOs 表现出比游离阿魏酸更高的抗氧化活性，DPPH 的 IC50 值为 11 vs 128 μM，ORAC 值为 4.77 vs 2.62 μmol Trolox/μmol。四唑蓝测定表明，添加 FA 浓度 > 50 μM 的 FO 显着提高了 H2O2 处理后的细胞活力。流式细胞术分析显示，添加浓度为800、200和50 μM的FOs可显着降低亚G0期细胞凋亡率，分别从37.5%降低至12.7%、16.2%和20.9%（P < 0.01）。 FO 还显着降低丙二醛含量和乳酸脱氢酶 (LDH) 活性，但增加用 H2O2 处理的 PC 12 细胞中的超氧化物歧化酶活性，并通过减少培养物中 LDH 的释放来防止细胞膜损伤。添加 800 μM FA 的效果与添加 200 μM FO 的效果相似。因此，由玉米麸皮制备的FO是对抗氧化应激的潜在功能成分。
Feruloylated oligosaccharides (FOs) were prepared by autoclaving corn bran in oxalic acid (0.6%) solution, and their protection effects against oxidative stress in pheochromocytoma cells (PC 12) cells were investigated. The FOs samples, which comprised a mixture of feruloylated mono- and dipentoses with 4.88% bound ferulic acid (FA), as well as xylose, arabinose, galactose, and glucose amounting to 46.43, 40.46, 3.76, and 8.68% of the total sugars, respectively, were prepared by autoclaving the pretreated corn bran in 0.6% oxalic acid and then further separated. Antioxidant activity was tested by 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) scavenging and oxygen radical absorbance capacity (ORAC) methods. Oxidative stress was induced by H2O2 in PC 12 neuronal cell culture model. The results showed that FOs exhibited higher antioxidant activity than free ferulic acid, with an IC50 value of 11 versus 128 μM for DPPH and an ORAC value of 4.77 versus 2.62 μmol Trolox/μmol. Tetrazolium blue assay showed that the addition of FOs with an FA concentration >50 μM significantly increased cell viability after treatment with H2O2. Flow cytometry analysis showed that the addition of FOs at concentrations of 800, 200, and 50 μM significantly decreased the apoptosis rate at the sub-G0 phase from 37.5 to 12.7, 16.2, and 20.9% (P < 0.01), respectively. FOs also significantly decreased the malonic dialdehyde content and lactate dehydrogenase (LDH) activity, but increased superoxide dismutase activity in PC 12 cells treated with H2O2 and prevented the damage of cellular membranes by decreasing the release of LDH to the cultures. The addition of FA at 800 μM showed an effect similar to that of FOs at 200 μM. Therefore, the FOs prepared from corn bran are potential functional ingredients for protection against oxidative stress.