Increased ribosomal biogenesis induces pancreatic β cell failure in mice model of type 2 diabetes
Increased ribosomal biogenesis induces pancreatic β cell failure in mice model of type 2 diabetes
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DOI：
10.1016/j.bbrc.2009.02.047
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发表时间：
2009-04-10
影响因子：
3.1
通讯作者：
Kasuga, Masato
中科院分区：
文献类型：
--
作者：
Asahara, Shun-ichiro;Matsuda, Tomokazu;Kasuga, Masato
目的：研究胰岛素抵抗条件下胰岛β细胞基因表达的变化。方法：利用肥胖糖尿病模型Lepr I小鼠分离的胰岛，进行All穷举基因表达分析。结果：通过对db/db小鼠胰岛的基因表达分析，我们发现核糖体相关分子的表达显著增加。此外，在一定的蛋白质水平上，细胞周期蛋白D2的表达也有所增加。当INS-1细胞被诱导过表达细胞周期蛋白D2时，我们发现核糖体相关分子的表达增加。同时还发现内质网应激相关分子表达增加。结论：在胰岛素抵抗相关性胰腺p细胞增生症中，核糖体生物合成增加，并诱导内质网应激。(C)2009 Elsevier Inc.保留所有权利。
Aim: To Study the changes in gene expression by pancreatic beta cells under insulin resistance conditions. Method: Ail exhaustive gene expression analysis was performed, Using isolated pancreatic islets of obese diabetic model Lepr I mice. Overexpression of cyclin D2 was induced in cells from the pancreatic beta cell line, namely, INS-1.Results: Through a gene expression analysis using islets isolated from db/db mice, we found a significant increase in the expression of ribosome-related molecules. In addition, increased expression of cyclin D2 was found at certain protein levels. As INS-1 cells were induced to overexpress cyclin D2, We found an increase in the expression of ribosome-related molecules. Concurrently, an increase in the expression of endoplasmic reticulum stress (ER stress)-related molecules was also found.Conclusion: in cases of pancreatic p cell hyperplasia associated with insulin resistance, ribosomal biogenesis is increased, and ER stress is induced. (C) 2009 Elsevier Inc. All rights reserved.