Systematic investigation of in vitro and in vivo safety, toxicity and degradation of mesoporous silica nanoparticles synthesized using commercial sodium silicate
Systematic investigation of in vitro and in vivo safety, toxicity and degradation of mesoporous silica nanoparticles synthesized using commercial sodium silicate
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DOI：
10.1016/j.micromeso.2019.04.050
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发表时间：
2019-08-01
影响因子：
5.2
通讯作者：
Sawant, Krutika
中科院分区：
文献类型：
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作者：
Bhaysar, Dhaval;Patel, Vijay;Sawant, Krutika
介孔氧化硅纳米粒子（MSNs）由于其独特的性质而被广泛研究作为药物递送载体。尽管使用非常经济的二氧化硅来源硅酸钠合成的MSN是有前途的药物递送载体，但关于其生物相容性和体内毒性的信息很少。本文研究了以工业硅酸钠为原料合成的介孔二氧化硅纳米粒子的生物相容性、毒性和降解性能。体外降解研究证实，MSN在PBS 7中6天内降解为柠檬酸。4发现合成的MSN与不同的细胞系如人胚肾（HEK-293）细胞、人高加索结肠腺癌（Caco-2）细胞、人肝癌（HepG 2）细胞和小鼠成纤维细胞（3 T3）细胞在200 μ g/mL浓度下细胞活力大于90%。当与鸡红细胞孵育2小时时，它们在高达200 μ g/mL的浓度下不引起显著的溶血（仅1.87%）。此外，血液样品的血液学和生物化学试验以及MSN注射后组织的组织病理学检查证实，小鼠对高达40 mg/kg的MSN耐受性良好。注射的MSNs在4天内完全通过大鼠（静脉注射20 mg/kg MSNs）的尿液和粪便以甘草酸的形式排出。
Mesoporous silica nanoparticles (MSNs) have been extensively investigated as drug delivery carriers due to their unique properties. Although MSNs synthesized using a very economic source of silica, sodium silicate, are promising carriers for drug delivery, information regarding their biocompatibility and in vivo toxicity is scarce. In the present work, biocompatibility, toxicity profile and degradation of mesoporous silica nanoparticles synthesized using commercial sodium silicate have been studied. In vitro degradation studies confirmed that MSNs got degraded to silicic acid within 6 days in PBS 7.4 The synthesized MSNs were found to be biocompatible to different cell lines such as human embryonic kidney (HEK-293) cells, human caucasian colon adenocarcinoma (Caco-2) cells, human liver carcinoma (HepG2) cells and mouse fibroblast (3T3) cells with greater than 90% cell viability at concentration of 200 mu g/mL. They did not cause significant hemolysis (only 1.87%) up to 200 mu g/mL concentration when incubated for 2 h with chicken red blood cells. Furthermore, hematological and biochemical tests of blood samples as well as histopathological examinations of tissues after MSN injection, confirmed that the MSNs were well tolerated by mice up to 40 mg/kg. Injected MSNs were completely excreted out through urine and feces by rats (injected intravenously with 20 mg/kg of MSNs) within 4 days in form of silicic acid.