Structural and functional changes in cerebral arteries from spontaneously hypertensive rats.
Structural and functional changes in cerebral arteries from spontaneously hypertensive rats.
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DOI：
10.1161/01.hyp.5.3.292
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发表时间：
1983-05
期刊：
影响因子：
8.3
通讯作者：
Raymond J. Winquist;Raymond J. Winquist
中科院分区：
文献类型：
--
作者：
Raymond J. Winquist;Raymond J. Winquist
应用微血管装置对自发性高血压大鼠（SHR）和正常血压大鼠（WKY）的基底动脉段进行了体外研究。在类似水平的被动力量，SHR的基底动脉开发更少的力量响应去极化溶液（130 mM K+）相比，基底动脉从WKY。来自高血压动物的动脉段需要较少的拉伸来实现每个水平的被动力。SHR而非WKY的基底动脉通常表现出阶段性和强直性自发活动，其通过在不添加Ca++的生理盐溶液（EGTA，1 mM）中洗涤组织以可逆的方式被抑制。与WKY相比，SHR基底动脉中5-羟色胺的EC 50显著左移，对该激动剂的最大反应更大（p <0.01）。与正常血压对照组相比，SHR动脉中Ca++（加入去极化溶液）的EC 50向右移动（p <0.05）。两组动物的动脉在异丙肾上腺素产生的舒张反应方面没有差异。然而，收缩的SHR的基底动脉对升高的Ca++的舒张作用的敏感性低于收缩的WKY的基底动脉（p <0.05）。这些结果表明SHR和WKY脑血管在结构和功能上存在差异。我们的研究结果也表明了高血压动物血管中钙动力学变化的复杂性。
Segments of basilar arteries from both spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats were studied in vitro utilizing a microvessel apparatus. At similar levels of passive force, basilar arteries from SHR developed less force in response to depolarizing solution (130 mM K+) compared to basilar arteries from WKY. Arterial segments from the hypertensive animals required less stretch to achieve each level of passive force. Basilar arteries from SHR but not WKY typically displayed both phasic and tonic spontaneous activity which was inhibited in a reversible manner by washing the tissues in physiological salt solution without added Ca++ (EGTA, 1 mM). There was a significant shift to the left in the EC50 of serotonin and a greater maximal response to this agonist in basilar arteries from SHR compared to those from WKY (p less than 0.01). The EC50 to Ca++ (added to a depolarizing solution) was shifted to the right in the arteries from SHR compared to the normotensive controls (p less than 0.05). There was no difference between the arteries from the two groups of animals in the relaxation response produced by isoproterenol. However, contracted basilar arteries from SHR were less sensitive to the relaxant effects of elevated Ca++ than contracted basilar arteries from WKY (p less than 0.05). These results demonstrate the existence of both structural and functional difference between cerebral vessels of SHR and WKY. Our findings also demonstrate the complex nature of the changes in calcium dynamics in blood vessels from hypertensive animals.