The role of monocyte chemoattractant protein-1 in experimental chronic pancreatitis model induced by dibutyltin dichloride in rats.
The role of monocyte chemoattractant protein-1 in experimental chronic pancreatitis model induced by dibutyltin dichloride in rats.
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DOI：
10.1097/00006676-200211000-00023
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发表时间：
2002-11-01
期刊：
影响因子：
2.9
通讯作者：
Nawata, Hajime
中科院分区：
文献类型：
--
作者：
Inoue, Masanobu;Ino, Yoshifumi;Nawata, Hajime
简介：最近报道二丁基二氯化锡（DBTC）在28天内诱导大鼠胰腺纤维化，但尚不清楚诱导的情况是否应被视为慢性胰腺炎。 目的和方法：本研究的目的是阐明DBTC诱导的胰腺纤维化是否可以被视为慢性胰腺炎。此外，我们在该模型中研究了单核细胞趋化蛋白-1 (MCP-1) 与胰腺纤维化发展的关系。将DBTC溶液注入大鼠右颈静脉，在第1、3、7、14和28天测量生化和组织学变化。结果：镜下观察，第1和3天胰腺中有明显炎性细胞浸润，第7、14和28天观察到单核细胞浸润，7天后胰腺纤维化明显。第28天，间质纤维化以及腺体和导管样管状复合体萎缩。 DBTC 使胰腺蛋白和淀粉酶的含量显着降低，而胰腺羟脯氨酸的含量则增加。与对照组相比，血清和胰腺MCP-1浓度显着升高。此外，MCP-1升高后，胰腺中PDGF mRNA的表达增加。结论：这些结果表明，DBTC诱导的大鼠胰腺纤维化实验模型可用作慢性胰腺炎模型，并且MCP-1可能在胰腺纤维化的发展中发挥重要作用。
INTRODUCTION: Recently, dibutyltin dichloride (DBTC) was reported to induce pancreatic fibrosis within 28 days in rats, but it is not clear that the induced condition should be considered chronic pancreatitis.AIM AND METHODOLOGY: The aim of this study was to clarify whether the pancreatic fibrosis induced by DBTC can be regarded as chronic pancreatitis. Furthermore, we examined the relation of monocyte chemoattractant protein-1 (MCP-1) to the development of pancreatic fibrosis in this model. DBTC solution was injected into the right jugular vein in rats, and biochemical and histologic changes were measured at days 1, 3, 7, 14, and 28.RESULTS: Microscopically, inflammatory cell infiltration was evident in the pancreas at days 1 and 3, mononuclear cell infiltration was observed at days 7, 14, and 28, and pancreatic fibrosis was pronounced 7 days later. At day 28, interstitial fibrosis and atrophy of the gland and ductlike tubular complex had progressed. DBTC produced a significant decrease in the contents of pancreatic protein and amylase, whereas the pancreatic hydroxyproline content increased. Serum and pancreatic MCP-1 concentration significantly increased compared with the control group. Furthermore, the expression of PDGF mRNA in the pancreas increased following the MCP-1 elevation.CONCLUSIONS: These results suggest that this experimental model of pancreatic fibrosis induced by DBTC in rats was useful as a chronic pancreatitis model and that MCP-1 may play an important role in the development of pancreatic fibrosis.