TRP Channel Reconstitution in Lipid Bilayers.
TRP Channel Reconstitution in Lipid Bilayers.
复制标题
脂质双层中的 TRP 通道重建。
DOI：
10.1007/978-1-4939-9446-5_10
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Zakharian,Eleonora
中科院分区：
文献类型：
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作者：
Zakharian,Eleonora
瞬时受体电位 (TRP) 蛋白家族呈现出多种多模式离子通道，它们在各种生理过程的调节中相互交织。目前，TRP 通道在温度感觉、体温调节、痛觉和矿物质稳态方面已很成熟。此外，新的证据表明，TRP 通道还与激素信号传导有关，这些通道负责沿着神经回路传播激素诱导的信号，并调节非兴奋细胞的细胞过程。由于其广泛的作用，TRP 通道在各个领域引起了巨大的科学兴趣。在本章中，我使用称为平面脂质双层的电生理学方法描述了几种 TRP 通道的掺入和表征。该技术的特点是测量明确的重构系统中离子通道的功能活动。这种电生理学方法的首要任务是识别离子通道的内在特性，这对于了解与thermo-TRP通道有关的内在温度敏感性特别有价值，而且还可以了解通道激动剂、拮抗剂、辅因子和其他调节剂的直接机制。
The family of the transient receptor potential (TRP) proteins presents a diverse group of polymodal ion channels intertwined in the regulation of various physiological processes. Currently, TRP channels are well established in temperature-sensation, thermoregulation, pain sensation, and mineral homeostasis. Furthermore, new evidence suggests that TRP channels are also implicated in hormonal signaling, where the channels are responsible for propagating hormone-induced signals along the neural circuitry and also regulating cellular processes of nonexcitable cells. Due to this wide assortment of actions, TRP channels have been attracting immense scientific interest in various fields.In this chapter, I describe incorporation and characterization of several TRP channels using an electrophysiological approach known as planar lipid bilayers. This technique features measurements of functional activities of ion channels in a well-defined reconstituted system. The priority of this electrophysiological approach is identifying intrinsic properties of ion channels, which is particularly valuable in appreciating intrinsic temperature sensitivity concerning thermo-TRP channels, but also direct mechanisms of channels agonists, antagonists, cofactors, and other modifiers.