Selectivity control in micellar electrokinetic chromatography of small peptides using mixed fluorocarbon-hydrocarbon anionic surfactants.
Selectivity control in micellar electrokinetic chromatography of small peptides using mixed fluorocarbon-hydrocarbon anionic surfactants.
复制标题
使用混合氟碳烃阴离子表面活性剂对小肽进行胶束电动色谱的选择性控制。
DOI：
10.1016/0021-9673(94)01027-c
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Khaledi,MG
中科院分区：
文献类型：
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作者：
Ye,B;Hadjmohammadi,M;Khaledi,MG
使用氟碳阴离子表面活性剂全氟辛烷磺酸锂和碳氢阴离子表面活性剂十二烷基硫酸锂的混合物，通过胶束电动色谱（MEKC）测定了一组含Trp小肽的电泳迁移率和容量因子。在混合这两种具有不同微环境和相互作用特性的表面活性剂时，实现了对溶质迁移的更大控制。混合胶束组成的变化，如表面活性剂的摩尔分数的变化，导致不同的溶质-胶束结合以及胶束的迁移时间（tmc）。因此，容量因子、选择性和洗脱窗口（tmc/t0）随混合胶束体系的组成而变化。碳氟化合物-烃表面活性剂的混合物的另一个特征是形成两种不同类型的胶束的可能性，这为MEKC系统中的每种溶质提供了额外的分配过程。这种独特的现象提供了更高程度的选择性控制。这种混合的MEKC体系对于小分子肽的分离是相当有效的。它提供了一种替代自由溶液毛细管区带电泳系统的分离带电溶质具有几乎相同的电泳迁移率。
Electrophoretic mobilities and capacity factors for a group of Trp-containing small peptides were determined by micellar electrokinetic chromatography (MEKC) using mixtures of a fluorocarbon anionic surfactant, lithium perfluorooctane sulfonate, and a hydrocarbon anionic surfactant, lithium dodecyl sulfate. Upon mixing these two surfactants, which have different microenvironments and interactive characteristics, greater control over migration of solutes is achieved. The changes in the composition of mixed micelles such as the mole fraction of the surfactants result in different solute-micelle binding as well as migration times of the micelles (tmc). Consequently, capacity factor, selectivity and elution window (tmc/t0) change with the composition of the mixed micellar system. Another characteristic of the mixtures of fluorocarbon-hydrocarbon surfactants is the possibility of forming two different types of micelles which offers an additional partitioning process for each solute in the MEKC system. Such a unique phenomenon offers a higher degree of selectivity control. This mixed MEKC system is quite effective for the separation of small peptides. It provides an alternative to the free-solution capillary zone electrophoresis system for the separation of charged solutes with nearly identical electrophoretic mobility.