Characterization of murine hemopoietic cells using rat anti-mouse monoclonal antibodies.
Characterization of murine hemopoietic cells using rat anti-mouse monoclonal antibodies.
复制标题
使用大鼠抗小鼠单克隆抗体表征小鼠造血细胞。
DOI：
10.1089/hyb.1983.2.55
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Goldwasser,E
中科院分区：
文献类型：
--
作者：
Loken,MR;Dessner-DeJose,DS;VanZant,G;Goldwasser,E
与小鼠骨髓中不同细胞群反应的三种大鼠抗小鼠单克隆抗体与淋巴造血系统中细胞的结合已被广泛表征。一种抗体鉴定专门用于B谱系的淋巴细胞。第二种抗体鉴定骨髓谱系的细胞，包括粒细胞和巨噬细胞。第三种抗体结合几乎所有的骨髓细胞，包括红细胞，然而，抗原表达的定量差异允许区分早期和晚期红细胞，并可用于纯化嗜酸性粒细胞。这三种单克隆抗体与成熟细胞、未成熟细胞和祖细胞（CFU-S、CFU-C（GM）、BFU-E和CFU-E）的差异结合允许构建基于三种细胞表面抗原表达的淋巴造血分化方案。
Three rat anti-mouse monoclonal antibodies reactive with different populations of cells in mouse bone marrow have been extensively characterized with respect to their binding to cells in the lymphohemopoietic system. One antibody identifies lymphocytes committed exclusively to the B lineage. A second antibody identifies cells of the myeloid lineage including granulocytes and macrophages. The third antibody binds to nearly all of the bone marrow cells including erythrocytes, however, quantitative differences in antigenic expression allows the discrimination between early and late erythroid cells and can be used to purify eosinophils. The differential binding of these three monoclonal antibodies to mature cells, immature cells and progenitor cells (CFU-S, CFU-C (GM), BFU-E, and CFU-E) permits the construction of a scheme of lymphohemopoietic differentiation based on the expression of three cell surface antigens.