Immunohistochemical and scanning electron microscopic comparison of the collagen network constructions between pig, goat and chicken livers
Immunohistochemical and scanning electron microscopic comparison of the collagen network constructions between pig, goat and chicken livers
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DOI：
10.1111/j.1740-0929.2009.00660.x
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发表时间：
2009-01-01
影响因子：
2
通讯作者：
Tabata, Shoji
中科院分区：
文献类型：
--
作者：
Nishimura, Shotaro;Sagara, Ayano;Tabata, Shoji
比较了猪、羊和鸡肝脏胶原纤维的分布和三维结构。免疫组化染色显示，I型胶原被确定在小叶间结缔组织区和小叶内地区的猪和山羊。在小叶间结缔组织区域和小叶内窦壁中也发现了III型胶原。在鸡肝中，只有血管的圆周区域用I型和III型胶原抗体免疫染色，小叶间结缔组织壁不能清楚区分。在小叶内，I型胶原抗体免疫反应在肝细胞周围，而III型胶原抗体免疫反应较弱。在氢氧化钠浸渍标本中，发达的胶原束形成了猪突出的小叶间壁。相比之下，山羊肝脏中的壁由一层薄薄的纤维束组成。鸡肝小叶间无明显胶原分隔。小叶内胶原结构在动物之间存在很大差异，表明猪中的胶原纤维网络脆弱，山羊中的框架坚固，鸡中的隔膜致密。这些结果表明，不同的胶原框架可能有助于每种动物肝脏的组织学强度。
The distribution and three-dimensional architecture of collagen fibers were compared between pig, goat and chicken livers. Immunohistochemical staining revealed that collagen type I was identified in the interlobular connective tissue region and intralobular areas in pigs and goats. Type III collagen was also identified in the interlobular connective tissue region and intralobular sinusoidal walls. In the chicken liver, only the circumference region of the vessels was immunostained with collagen type I and III antibodies and the interlobular connective tissue wall could not be distinguished clearly. In the intralobular region, collagen type I antibody immunoreacted around the hepatic cells but collagen type III antibody immunoreacted weakly. In the NaOH macerated specimen, well-developed collagen bundles formed the prominent interlobular walls in pigs. In contrast, the wall in the goat liver comprised a thin layer of the bundles. In the chicken liver, there were no notable collagen septa between lobules. The intralobular collagen construction was quite different between the animals, indicating a fragile collagen fibril networks in pigs, a robust framework in goats and dense fabric-like septa in chickens. These results indicate that the distinct collagen frameworks may contribute to the histological strength of the livers in each of the animal species.