Extracting Mural and Volumetric Growth Patterns of Platelet Aggregates on Engineered Surfaces by Use of an Entity Tracking Algorithm.
Extracting Mural and Volumetric Growth Patterns of Platelet Aggregates on Engineered Surfaces by Use of an Entity Tracking Algorithm.
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DOI：
10.1097/mat.0000000000001841
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发表时间：
2023-04-01
期刊：
影响因子：
4.2
通讯作者：
Kirby, Brian J.
中科院分区：
文献类型：
--
作者：
Kang, Junhyuk;Jayaraman, Anjana;Antaki, James F.;Kirby, Brian J.
血栓形成是一种主要并发症，可能发生在血液接触装置和区域以及血管损伤区域。微流体装置是流行的模板，用于模拟各种血栓形成环境并评估导致整体通道闭塞的条件。然而，面积平均测量错过了提取有关血栓演化以及血栓形成和传播的早期动态的实时信息的机会，从而导致晚期整体通道闭塞。为了阐明这些动态，我们开发了一种独立的跟踪算法，该算法使用连续图像连接性和最小质心距离映射来唯一索引荧光延时视频补充视频 1 和补充视频 2 中所有出现的血栓。这导致了对所有单独聚合体的测量，这些聚合体又可以作为整体进行研究。我们将跟踪应用于荧光延时视频补充视频 1 和补充视频 2，显示剪切速率为 4000 s−1 时胶原功能化基底和粗糙钛合金 (Ti6Al4V) 表面的血栓形成情况。当将整体平均测量值与面积平均指标进行比较时，我们揭示了胶原蛋白表面上早期阶段的即时、稳定的血栓生长以及 Ti6Al4V 表面上粗糙 Ti6Al4V 表面上不稳定的血栓附着。此外，我们引入了跟踪的血栓偏心率和荧光强度作为血栓生长的附加体积测量，其与起作用的主要血栓形成机制相关。这项工作主张将表面宏观状态指标与特征性血栓微观状态生长模式相补充，以准确预测关键的血栓形成事件。
Thrombosis is a major complication that can occur in both blood-contacting devices and regions and in regions of vascular damage. Microfluidic devices are popular templates to model various thrombogenic settings and to assess conditions that lead to bulk channel occlusion. However, area-averaged measurements miss the opportunity to extract real-time information on thrombus evolution and early dynamics of thrombus formation and propagation, which result in late-stage bulk channel occlusion. To clarify these dynamics, we have developed a standalone tracking algorithm that uses consecutive image connectivity and minimal centroid distance mappings to uniquely index all appearing thrombi in fluorescence time-lapse videos Supplemental Video 1, and Supplemental Video 2. This leads to measurements of all individual aggregates that can in turn be studied as ensembles. We applied tracking to fluorescence time-lapse videos Supplemental Video 1, and Supplemental Video 2 of thrombosis across both collagen-functionalized substrate and across the surface of a roughened titanium alloy (Ti6Al4V) at a shear rate of 4000 s−1. When comparing ensemble-averaged measurements to area-averaged metrics, we unveil immediate, steady thrombus growth at early phases on collagen surfaces and unstable thrombus attachment to roughened Ti6Al4V surfaces on Ti6Al4V surfaces. Additionally, we introduce tracked thrombus eccentricity and fluorescence intensity as additional volumetric measures of thrombus growth that relate back to the primary thrombosis mechanism at play. This work advocates for the complementation of surface macrostate metrics with characteristic thrombus microstate growth patterns to accurately predict critical thrombosis events.