Improvement of Distribution and Osteogenic Differentiation of Human Mesenchymal Stem Cells by Hyaluronic Acid and β-Tricalcium Phosphate-Coated Polymeric Scaffold In Vitro.
Improvement of Distribution and Osteogenic Differentiation of Human Mesenchymal Stem Cells by Hyaluronic Acid and β-Tricalcium Phosphate-Coated Polymeric Scaffold In Vitro.
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DOI：
10.1089/biores.2015.0021
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发表时间：
2015
影响因子：
--
通讯作者：
Lysdahl H
中科院分区：
文献类型：
--
作者：
Chen M;Le DQ;Kjems J;Bünger C;Lysdahl H
骨组织工程需要一种设计良好的支架，该支架既可生物降解，又具有生物相容性，并能支持干细胞向成骨分化。熔融沉积法制备多孔聚己内酯（PCL）支架是一种极具吸引力的生物材料，已广泛应用于临床。然而，PCL支架缺乏生物学功能和骨诱导能力。在本研究中，我们用透明质酸/β-磷酸三钙（HA/TCP）基质包埋PCL支架，使其功能化。将人间充质干细胞（MSCs）培养在有涂层和无涂层的支架上，研究其增殖和成骨分化。在第21天，HA/ tcp包被支架的DNA量显著增加。在基因表达水平上，HA/TCP包被在第4天显著提高了ALP和COLI的表达。这些数据与HA/ tcp包被支架的ALP活性在第7天达到峰值相关。扫描电镜和组织学分析显示，与未包被支架相比，包被支架内细胞基质和钙沉积分布更均匀。综上所述，HA/TCP涂层促进了体外细胞增殖、成骨分化和细胞基质均匀分布。HA/TCP-PCL支架很有希望容纳人骨髓来源的MSCs用于骨重建目的，这值得未来的体内研究。
Bone tissue engineering requires a well-designed scaffold that can be biodegradable, biocompatible, and support the stem cells to osteogenic differentiation. Porous polycaprolactone (PCL) scaffold prepared by fused deposition modeling is an attractive biomaterial that has been used in clinic. However, PCL scaffolds lack biological function and osteoinductivity. In this study, we functionalized the PCL scaffolds by embedding them with a matrix of hyaluronic acid/β-tricalcium phosphate (HA/TCP). Human mesenchymal stem cells (MSCs) were cultured on scaffolds with and without coating to investigate proliferation and osteogenic differentiation. The DNA amount was significantly higher in the HA/TCP-coated scaffold on day 21. At the gene expression level, HA/TCP coating significantly increased the expression of ALP and COLI on day 4. These data correlated with the ALP activity peaking on day 7 in the HA/TCP-coated scaffold. Scanning electron microscope and histological analysis revealed that the cell matrix and calcium deposition were distributed more uniformly in the coated scaffolds compared to scaffolds without coating. In conclusion, the HA/TCP coating improved cellular proliferation, osteogenic differentiation, and uniform distribution of the cellular matrix in vitro. The HA/TCP-PCL scaffold holds great promise to accommodate human bone marrow-derived MSCs for bone reconstruction purposes, which warrants future in vivo studies.