Maternal Epigenetic Pathways Control Parental Contributions to Arabidopsis Early Embryogenesis
Maternal Epigenetic Pathways Control Parental Contributions to Arabidopsis Early Embryogenesis
复制标题
DOI：
10.1016/j.cell.2011.04.014
复制
发表时间：
2011-05-27
期刊：
影响因子：
64.5
通讯作者：
Grossniklaus, Ueli
中科院分区：
文献类型：
--
作者：
Autran, Daphne;Baroux, Celia;Grossniklaus, Ueli
定义胚胎发育过程中父本和母本基因组的贡献和相互作用对于理解杂种活力、杂种不育和生殖隔离的基本过程至关重要。为了确定亲本在拟南芥胚胎发生过程中的贡献及其调控，我们结合了基于深度测序的RNA谱分析和遗传分析。在2-4个细胞阶段，尽管转录本由亲本基因组贡献，但母体转录本在全基因组范围内具有很强的优势。在球形阶段，父系的贡献相对较高，主要是由于父系基因组的逐渐激活。我们确定了控制这些亲代贡献的两种拮抗母系途径。父本等位基因最初通过染色质siRNA途径下调，与DNA和组蛋白甲基化相关，而转录激活需要母本组蛋白伴侣复合物CAF1的活性。我们的研究结果定义了控制亲本在植物胚胎中的贡献的母系表观遗传途径，这与调节基因组印迹的途径不同。
Defining the contributions and interactions of paternal and maternal genomes during embryo development is critical to understand the fundamental processes involved in hybrid vigor, hybrid sterility, and reproductive isolation. To determine the parental contributions and their regulation during Arabidopsis embryogenesis, we combined deep-sequencing-based RNA profiling and genetic analyses. At the 2-4 cell stage there is a strong, genome-wide dominance of maternal transcripts, although transcripts are contributed by both parental genomes. At the globular stage the relative paternal contribution is higher, largely due to a gradual activation of the paternal genome. We identified two antagonistic maternal pathways that control these parental contributions. Paternal alleles are initially downregulated by the chromatin siRNA pathway, linked to DNA and histone methylation, whereas transcriptional activation requires maternal activity of the histone chaperone complex CAF1. Our results define maternal epigenetic pathways controlling the parental contributions in plant embryos, which are distinct from those regulating genomic imprinting.