Nanofluidics for sub-single cellular studies: Nascent progress, critical technologies, and future perspectives
Nanofluidics for sub-single cellular studies: Nascent progress, critical technologies, and future perspectives
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DOI：
10.1016/j.cclet.2021.09.066
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发表时间：
2021-09
影响因子：
9.1
通讯作者：
Jinbin Yang;Yan Xu
中科院分区：
文献类型：
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作者：
Jinbin Yang;Yan Xu
在细胞研究领域，从单细胞水平进一步缩小到亚单细胞水平的研究是一个新兴的趋势。亚细胞物质是细胞的基本成分，与细胞异质性有关。亚单细胞研究主要关注单个细胞中的亚细胞物质，旨在从亚细胞物质甚至单组分/囊泡/分子水平上了解单个细胞的细节和异质性。因此，亚单细胞研究可以为基础细胞生物学和新的诊断和治疗技术和应用的发展提供更深入的见解。尽管如此，单个细胞的内容物不仅体积极小，而且组成极其复杂，远远超出了当前细胞研究中使用的大多数工具的能力。我们相信，纳米流体在为亚单细胞研究提供理想工具方面具有巨大的潜力，不仅因为它们能够处理飞升/一升尺度的样品，还因为它们有可能在单组分/囊泡/分子水平上以高通量的方式操纵和分析亚细胞物质。在这篇综述中，我们总结了纳米流体在亚单细胞研究领域的努力，重点介绍了新兴的进展和有潜力克服技术瓶颈的关键技术。本文还讨论了与信息科学相结合的挑战和未来机遇。
In the field of cell studies, there is a burgeoning trend to further downscale the investigation from a single-cell level to a sub-single-cell level. Subcellular matter is the basic content in cells and correlates with cell heterogeneity. Sub-single cellular studies focus on the subcellular matter in single cells and aim to understand the details and heterogeneity of individual cells in terms of the subcellular matter or even at the single component/vesicle/molecule level. Hence, sub-single cellular studies can provide deeper insights into fundamental cell biology and the development of new diagnostic and therapeutic technologies and applications. Nonetheless, the contents of a single cell are not only ultra-small in volume but also extremely complex in composition, far exceeding the capabilities of most tools used in current cell studies. We believe that nanofluidics holds great potential in providing ideal tools for sub-single cellular studies, not only because of their capability to handle femtoliter/attoliter-scale samples, but also because of their possibility to manipulate and analyze subcellular matters at the single component/vesicle/molecule level in a high-throughput manner. In this review, we summarize the efforts in the field of nanofluidics for sub-single cellular studies, focusing on nascent progress and critical technologies that have the potential to overcome the technical bottlenecks. Some challenges and future opportunities to integrate with information sciences are also discussed.