A heat shock cognate 70 gene in the endoparasitoid, Pteromalus puparum, and its expression in relation to thermal stress
A heat shock cognate 70 gene in the endoparasitoid, Pteromalus puparum, and its expression in relation to thermal stress
复制标题
内寄生蜂 Pteromalus puparum 中的热休克同源 70 基因及其与热应激相关的表达
DOI：
10.5483/bmbrep.2008.41.5.388
复制
发表时间：
2008-05-31
期刊：
影响因子：
3.8
通讯作者：
Ye, Gong-yin
中科院分区：
文献类型：
--
作者：
Wang, Huan;Dong, Sheng-zhang;Ye, Gong-yin
从拟内源寄生物羽绒寄主（Pleromalus puparum）中分离到热休克同源基因Pphsc70，并对其进行了鉴定。该cDNA全长2204个碱基对（bp），包含一个1968 bp的ORF，编码656个氨基酸的多肽，预测分子量为71.28 kDa。基于15种昆虫Hsc70氨基酸序列的系统发育分析与目前的系统发育一致。此外，基因组DNA证实了位于编码区和5'UTR的三个内含子的存在。热处理后，Pphsc70表达显著升高，但持续热休克或恢复后，诱导mRNA表达逐渐下降，显著低于对照蛹（P < 0.05）。另外，经40℃预热、50℃保温1 h后，蛹的羽化率明显高于直接保温50℃的蛹，说明逐渐升高的温度可以增强昆虫的耐热性。
The Pphsc70 (heat shock cognate 70) gene was isolated from the endoparasitoid Pleromalus puparum and then characterized. The full-length cDNA was 2204 base pair (bp) and contained a single 1968 bp ORF that encoded a polypeptide of 656 amino acids with a predicted molecular mass of 71.28 kDa. Phylogenetic analysis based on Hsc70 amino acid sequences from fifteen insect species agreed with the present phylogeny. In addition, genomic DNA confirmed the presence of three introns located at the coding region as well as the 5'UTR. A significant elevation of Pphsc70 expression was observed following heat treatment, however, continued exposure to heat shock or recovery caused the expression of induced mRNA to gradually decline to levels that were significantly lower than those of control pupae (P < 0.05). In addition, a significant increase was observed in the emergence rate of pupae that were preheated at 40 degrees C and then exposed to 50 degrees C for I h when compared with the pupae that were not preheated, but instead directly exposed to 50 degrees C. Taken together, these results revealed that exposure to gradually increasing temperatures can enhance an insects thermo-tolerance.