VARIATION IN PROPORTION AND MOLECULAR-WEIGHT OF NATIVE CRYSTALLINS FROM SINGLE HUMAN LENSES UPON AGING AND FORMATION OF NUCLEAR CATARACT
VARIATION IN PROPORTION AND MOLECULAR-WEIGHT OF NATIVE CRYSTALLINS FROM SINGLE HUMAN LENSES UPON AGING AND FORMATION OF NUCLEAR CATARACT
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DOI：
10.1016/0014-4835(83)90137-9
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发表时间：
1983-01-01
影响因子：
3.4
通讯作者：
WOLLENSAK, J
中科院分区：
文献类型：
--
作者：
BESSEMS, GJH;HOENDERS, HJ;WOLLENSAK, J
用高压凝胶渗透色谱结合尺寸和浓度敏感检测器分析了人单个晶状体皮质和细胞核中的水溶性蛋白质提取物。用这种快速而准确的方法，观察了核性白内障形成和老化过程中天然晶状体蛋白的相对分子质量和比例的变化。在30到40岁的范围内，正常晶状体的γ-晶体蛋白含量增加，α-晶体蛋白的含量减少；40岁以上，皮质(11%)和细胞核(1-2%)的γ-晶体蛋白水平下降，α-晶体蛋白的水平保持不变。这些变化伴随着水不溶部分的增加。随着核性白内障的进展，β3-增加和β-晶体蛋白含量急剧下降，特别是在细胞核中，伴随着水不溶部分的急剧增加。晶状体蛋白的相对分子质量变化不大，但α-和β1-晶状体蛋白的相对分子质量随年龄增加而增加。
Extracts of water-soluble proteins from cortex and nucleus of single human lenses were analyzed by high-pressure gel permeation chromatography in combination with a size- and concentration-sensitive detector. Using this rapid and accurate method, changes in MW and proportion of the native crystallins upon aging and formation of nuclear cataract are observed. In the range from 30 to about 40 yr the .gamma.-crystallin content of normal lenses increases and that of .alpha.-crystallin decreases; over 40 yr the level of .gamma.-crystallin decreases and that of .alpha.-crystallin remains constant in the cortex (11%) as well as in the nucleus (1-2%). These changes are accompanied by an increase in the water-insoluble fraction. With progressing nuclear cataract an increase in .beta.3- and a drastic decrease in .gamma.-crystallin content, especially in the nucleus, are accompanied by a steep increase in the water-insoluble fraction. The MW of the crystallins do not change significantly, except that of .alpha.- and .beta.1-crystallin which show an increase with age.