Homoeologous recombination, chromosome engineering and crop improvement
Homoeologous recombination, chromosome engineering and crop improvement
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DOI：
10.1007/s10577-006-1108-8
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发表时间：
2007-01-01
影响因子：
2.6
通讯作者：
Gill, Bikram S.
中科院分区：
文献类型：
--
作者：
Qi, Lili;Friebe, Bernd;Gill, Bikram S.
Sears (1956) 于 50 年前开创了植物染色体工程，利用 X 射线照射和优雅的细胞遗传学方案将叶锈病抗性基因从外来染色体定向转移到小麦染色体。从那时起，许多其他方案被报道，但处理诱导同源配对和重组的方案是最强大的，并且已广泛用于小麦。在此，我们简要回顾了以小麦为重点的植物中基于同源重组的染色体工程研究现状，并证明细胞遗传库和分子资源的综合利用可以提高基于同源重组的染色体工程的效率和精度。我们报告了基于同源重组的病毒抗性从外来染色体转移到小麦染色体的实验结果、其表征以及通过第二轮重组进一步工程化的前景。提出了一项基于全基因组同源重组工程的提案，以有效挖掘野生近缘种的基因库以改良作物。
Sears ( 1956) pioneered plant chromosome engineering 50 years ago by directed transfer of a leaf rust resistance gene from an alien chromosome to a wheat chromosome using X-ray irradiation and an elegant cytogenetic scheme. Since then many other protocols have been reported, but the one dealing with induced homoeologous pairing and recombination is the most powerful, and has been extensively used in wheat. Here, we briefly review the current status of homoeologous recombination-based chromosome engineering research in plants with a focus on wheat, and demonstrate that integrated use of cytogenetic stocks and molecular resources can enhance the efficiency and precision of homoeologus-based chromosome engineering. We report the results of an experiment on homoeologous recombination-based transfer of virus resistance from an alien chromosome to a wheat chromosome, its characterization, and the prospects for further engineering by a second round of recombination. A proposal is presented for genome-wide, homoeologous recombination-based engineering for efficient mining of gene pools of wild relatives for crop improvement.