Proteomic analysis of BmN cells (Bombyx mori) in response to infection with Nosema bombycis
Proteomic analysis of BmN cells (Bombyx mori) in response to infection with Nosema bombycis
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DOI：
10.1093/abbs/gmu092
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发表时间：
2014-11-01
影响因子：
3.7
通讯作者：
Lu, Xingmeng
中科院分区：
文献类型：
--
作者：
He, Xinyi;He, Xiangkang;Lu, Xingmeng
家蚕微孢子虫（Nosema bombycis）N.家蚕微粒子病（Bombycis，Nb）是一种专性细胞内寄生虫，其可在家蚕中引起微粒子病。研究N. BmN细胞感染家蚕后的蛋白质组、感染和未感染家蚕后的BmN细胞的蛋白质组和未感染家蚕后的BmN细胞的蛋白质组。采用双向凝胶电泳和MALDI-TOF/TOF质谱技术对不同感染阶段的家蚕进行了鉴定，共鉴定出24个差异表达蛋白，在mock和N.家蚕感染的细胞。值得注意的是，基因本体分析表明，这些蛋白质参与许多重要的生物反应。在感染阶段，参与能量代谢和氧化应激的蛋白表达上调。两个参与泛素依赖蛋白分解代谢过程的蛋白表达下调。定量实时聚合酶链反应被用来分析这些确定的蛋白质的转录谱。总之，所鉴定的蛋白质的丰度变化、推定的功能和参与生物反应产生了N.家蚕感染的BmN细胞。这些发现进一步加深了我们对能量缺陷寄生虫对宿主细胞影响的认识。
Nosema bombycis (N. bombycis, Nb) is an obligate intracellular parasite, which can cause pebrine disease in the silkworm. To investigate the effects of N. bombycis infection on the host cells, proteomes from BmN cells that had or had not been infected with N. bombycis at different infection stages were characterized with two-dimensional gel electrophoresis and MALDI-TOF/TOF mass spectrometry, which identified 24 differentially expressed host proteins with significant intensity differences (P < 0.05) at least at one time point in mock- and N. bombycis infected cells. Notably, gene ontology analyses showed that these proteins are involved in many important biological reactions. During the infection phase, proteins involved in energy metabolism and oxidative stress had up-regulated expression. Two proteins participated in ubiquitin-dependent protein catabolic process had down-regulated expression. Quantitative real-time polymerase chain reaction was used to analyze the transcriptional profiles of these identified proteins. Taken together, the abundance changes, putative functions, and participation in biological reactions for the identified proteins produce a host-responsive protein model in N. bombycis-infected BmN cells. These findings further our knowledge about the effect of energy defect parasites on the host cells.