Effect of boldo (Peumus boldus Molina) infusion on lipoperoxidation induced by cisplatin in mice liver
Effect of boldo (Peumus boldus Molina) infusion on lipoperoxidation induced by cisplatin in mice liver
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DOI：
10.1002/ptr.2746
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发表时间：
2009-07-01
影响因子：
7.2
通讯作者：
Zamorano-Ponce, E.
中科院分区：
文献类型：
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作者：
Fernandez, J.;Lagos, P.;Zamorano-Ponce, E.
Peumus boldus Molina（Monimiaceae），通常被称为“boldo”，在传统的智利医学中用于治疗肝脏和胃肠道疾病。它的叶子富含抗氧化化合物，主要是生物碱和类黄酮。本研究评估了完整的博多叶输液对顺铂诱导的小鼠肝脏脂质过氧化（在532 nm处测定MDA）的保护作用。为了确定观察到的效果是否可以通过boldine或儿茶素的作用来解释，分别研究了每种化合物。将小鼠分为8组（n = 6）：（1）未治疗组，（11）顺铂治疗组。(III)（IV）用5% boldo叶输液预处理并用顺铂6 mg/Kg b.w.处理; (V)用boldine 50 mg/Kg b.w.处理; (VI)用boldine 50 mg/Kg b.w.预处理顺铂6 mg/kg体重; (VII)用儿茶素处理;和（VIII）用儿茶素50 mg/Kg b.w.顺铂6 mg/Kg b.w.正如预期的那样，与未治疗组相比，顺铂治疗显著增加了（p < 0.01）脂质过氧化。与未用该输注液预处理的动物相比，用博多叶输注液预处理显著降低了顺铂诱导的脂质过氧化作用（p < 0.05）。与仅用顺铂处理的组相比，用boldine和儿茶素预处理显着减少了顺铂诱导的脂质过氧化作用（p < 0.05）。结果表明，boldo输液是作为一种保护剂，相对于顺铂引起的氧化性肝损伤，这种保护能力将是由于天然抗氧化剂boldine和主要儿茶素的输液中的存在。这些发现表明，输液作为化学保护剂的潜在用途。版权所有（C）2009约翰威利父子有限公司
Peumus boldus Molina (Monimiaceae), commonly referred to as 'boldo', is used in traditional Chilean medicine to treat hepatic and gastrointestinal diseases. Its leaves are rich in antioxidant compounds, principally alkaloids and flavonoids. This study evaluates the protective effect of a complete boldo leaf infusion on lipoperoxidation (MDA determination at 532 nm) induced by cisplatin in mice liver. To determine if the observed effect can be explained by the action of boldine or catechin, each compound was studied separately. The mice were divided into 8 groups (n = 6): (1) not treated; (11) treated with cisplatin 6 mg/Kg b.w.; (III) treated with boldo leaf infusion 5%; (IV) pretreated with boldo leaf infusion 5% and treated with cisplatin 6 mg/Kg b.w.; (V) treated with boldine 50 mg/Kg b.w.; (VI) pretreated with boldine 50 mg/Kg b.w. and treated with cisplatin 6 mg/kg.b.w.; (VII) treated with catechin; and (VIII) pretreated with catechin 50 mg/Kg b.w. and treated with cisplatin 6 mg/Kg b.w. As expected, the treatment with cisplatin significantly increased (p < 0.01) lipoperoxidation in comparison with the non-treated group. Pretreatment with boldo leaf infusion significantly diminished (p < 0.05) the lipoperoxidation induced by cisplatin with respect to the animals not pretreated with the infusion. The pretreatments with boldine and catechin significantly diminished (p < 0.05) the lipoperoxidation induced by cisplatin with respect to the group treated only with cisplatin. The results suggest that the boldo infusion is acting as a protector with respect to the oxidative hepatic damage caused by cisplatin, and that this protective ability would be due to the presence in the infusion of the natural antioxidants boldine and principally catechin. These findings suggest the potential use of the infusion as a chemoprotector. Copyright (C) 2009 John Wiley & Sons, Ltd.