Candidate gene analysis of anthocyanin pigmentation loci in the Solanaceae
Candidate gene analysis of anthocyanin pigmentation loci in the Solanaceae
复制标题
DOI：
10.1007/s00122-003-1455-1
复制
发表时间：
2004-02-01
影响因子：
5.4
通讯作者：
Bodis, M
中科院分区：
文献类型：
--
作者：
De Jong, WS;Eannetta, NT;Bodis, M
茄科中的作物物种，包括番茄（Lycopersicon esculentum）、马铃薯（Solanum tuberosum）、胡椒（Capsicum spp.）和茄子（S. melongena），在花色素苷色素的类型、水平和组织特异性表达模式方面表现出天然变化。虽然这些作物中支持花青素性状自然变异的基因的身份在很大程度上是未知的，但已经从茄科观赏物种矮牵牛中分离出许多花青素生物合成的结构基因和调节因子。为了鉴定可能对应于控制四种作物自然变异的基因座的候选基因，将13个花青素相关基因定位在番茄F-2遗传图谱上。然后将基因图谱位置与番茄中的映射突变体进行比较，并通过比较遗传图谱与马铃薯、茄子和辣椒中的自然变异体进行比较。相似的图谱位置表明，番茄突变体花青素无，完全花青素，和花青素增益相应的类黄酮3 '5'-羟化酶（f3 '5'h），花青素合成酶，和矮牵牛Myb结构域转录调控基因an 2，分别。类似地，马铃薯R（产生红色天竺葵素基色素所需）、P（产生紫色飞燕草素基色素所需）和I（在块茎皮中组织特异性表达所需）似乎分别对应于二氢黄酮醇4-还原酶f3 ′ 5 ′ h和an 2。an 2的图谱位置也与辣椒A和茄子fap10.1、lla10.1、lra10.1、sa10.1、pa10.1和ca10.1重叠，表明在许多茄科作物的驯化过程中，一个同源的调控位点受到了平行选择。为了检验番茄无花色素苷对应于f3 '5'h的假设，对该基因的一部分进行测序。一个过早的终止密码子中观察到的花青素突变体，但不是在野生型。
Crop species in the Solanaceae, which includes tomato (Lycopersicon esculentum), potato (Solanum tuberosum), pepper (Capsicum spp.), and eggplant (S. melongena), exhibit natural variation in the types, levels, and tissue-specific expression patterns of anthocyanin pigments. While the identities of the genes underpinning natural variation in anthocyanin traits in these crops are largely unknown, many structural genes and regulators of anthocyanin biosynthesis have been isolated from the solanaceous ornamental species Petunia. To identify candidate genes that may correspond to loci controlling natural variation in the four crops, 13 anthocyanin-related genes were localized on a tomato F-2 genetic map. Gene map positions were then compared to mapped mutants in tomato and through comparative genetic maps to natural variants in potato, eggplant, and pepper. Similar map positions suggest that the tomato mutants anthocyaninless, entirely anthocyaninless, and anthocyanin gainer correspond to flavonoid 3'5'-hydroxylase (f3'5'h), anthocyanidin synthase, and the Petunia Myb domain trancriptional regulatory gene an2, respectively. Similarly potato R, required for the production of red pelargonidin-based pigments, P, required for production of purple delphinidin-based pigments, and I, required for tissue-specific expression in tuber skin, appear to correspond to dihydroflavonol 4-reductase, f3'5'h and an2, respectively. The map location of an2 also overlaps pepper A and eggplant fap10.1, lla10.1, lra10.1, sa10.1, pa10.1 and ca10.1, suggesting that a homologous regulatory locus has been subjected to parallel selection in the domestication of many solanaceous crops. To test the hypothesis that tomato anthocyaninless corresponds to f3'5'h, a portion of the gene was sequenced. A premature stop codon was observed in an anthocyaninless mutant, but not in wild-type.