Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia
Osteogenic proliferation and differentiation of canine bone marrow and adipose tissue derived mesenchymal stromal cells and the influence of hypoxia
复制标题
DOI：
10.1016/j.rvsc.2010.10.012
复制
发表时间：
2012-02-01
影响因子：
2.4
通讯作者：
Yellowley, Clare E.
中科院分区：
文献类型：
--
作者：
Chung, Dai-Jung;Hayashi, Kei;Yellowley, Clare E.
本研究的目的是比较犬骨髓间充质干细胞（BM-cMSCs）和脂肪组织间充质干细胞（AT-cMSCs）的成骨和增殖能力。在不同的氧分压（1%，5%和21%O-2）下测定增殖潜力。还研究了降低的氧水平对AT-cMSCs成骨分化的影响。AT-cMSCs的增殖速度明显快于BM-cMSCs，尽管两种细胞类型都显示出强大的成骨分化。在5%和1%O-2中培养会损害两种来源的cMSC的增殖和AT-cMSC的成骨分化。我们的数据表明，AT-cMSCs可能更适合用于临床情况，其中骨修复需要大量细胞，因为它们的快速增殖与强大的成骨潜力相结合。我们的数据还表明，缺氧对细胞增殖和分化的抑制作用时，应考虑使用骨髓间充质干细胞在一个潜在的缺氧环境，如骨折部位。(C)2010爱思唯尔有限公司版权所有。
The aim of this study was to compare the osteogenic and proliferative potential of canine mesenchymal stromal cells (cMSCs) derived from bone marrow (BM-cMSCs) and adipose tissue (AT-cMSCs). Proliferation potential was determined under varying oxygen tensions (1%, 5%, and 21% O-2). Effects of reduced oxygen levels on the osteogenic differentiation of AT-cMSCs were also investigated. AT-cMSCs proliferated at a significantly faster rate than BM-cMSCs, although both cell types showed robust osteogenic differentiation. Culture in 5% and 1% O-2 impaired proliferation in cMSC from both sources and osteogenic differentiation in AT-cMSCs. Our data suggests that AT-cMSCs might be more suitable for use in a clinical situation, where large cell numbers are required for bone repair, due to their rapid proliferation combined with robust osteogenic potential. Our data also suggests that the inhibitory effects of hypoxia on both cell proliferation and differentiation should be considered when using MSCs in a potentially hypoxic environment such as a fracture site. (C) 2010 Elsevier Ltd. All rights reserved.