Tissue imaging depth limit of stimulated Raman scattering microscopy
Tissue imaging depth limit of stimulated Raman scattering microscopy
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DOI：
10.1364/boe.382396
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发表时间：
2020-02-01
影响因子：
3.4
通讯作者：
Fu, Dan
中科院分区：
文献类型：
--
作者：
Hill, Andrew H.;Manifold, Bryce;Fu, Dan
受激拉曼散射（SRS）显微镜是一种很有前途的技术，用于研究组织结构，生理和功能。与其他非线性光学成像技术类似，由于组织的混浊和异质性，SRS在成像深度方面受到严重限制，无论是在透射模式还是在epi模式下成像。虽然这一挑战是众所周知的，重要的成像参数（即最大成像深度和成像信噪比）很少在文献中报道。比较epi模式和透射模式成像以确定用于许多组织成像应用的最佳几何结构也是重要的。在这篇手稿中，我们报告了可实现的信号大小和成像深度，同时使用epi/透射成像方法在四个不同的小鼠组织;脑，肺，肾，肝。对于所有四种情况下，我们报告的最大信号大小，散射长度和可实现的成像深度作为组织类型和样品厚度的函数。我们报告说，对于小鼠大脑样品薄于2毫米透射成像提供了更好的结果，而样品2毫米和更厚的最佳成像与epi成像。我们还演示了使用基于CNN的去噪算法，以产生40 μ m（24%）的可实现的成像深度增加。(C)根据OSA开放获取出版协议的条款，2020年美国光学学会
Stimulated Raman scattering (SRS) microscopy is a promising technique for studying tissue structure, physiology, and function. Similar to other nonlinear optical imaging techniques, SRS is severely limited in imaging depth due to the turbidity and heterogeneity of tissue, regardless of whether imaging in the transmissive or epi mode. While this challenge is well known, important imaging parameters (namely maximum imaging depth and imaging signal to noise ratio) have rarely been reported in the literature. It is also important to compare epi mode and transmissive mode imaging to determine the best geometry for many tissue imaging applications. In this manuscript we report the achievable signal sizes and imaging depths using a simultaneous epi/transmissive imaging approach in tour different murine tissues; brain, lung, kidney, and liver. For all four cases we report maximum signal sizes, scattering lengths, and achievable imaging depths as a function of tissue type and sample thickness. We report that for murine brain samples thinner than 2 mm transmissive imaging provides better results, while samples 2 mm and thicker are best imaged with epi imaging. We also demonstrate the use of a CNN-based denoising algorithm to yield a 40 mu m (24%) increase in achievable imaging depth. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement