Effect of sodium ferulate on the hyperalgesia mediated by P2X3 receptor in the neuropathic pain rats
Effect of sodium ferulate on the hyperalgesia mediated by P2X3 receptor in the neuropathic pain rats
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DOI：
10.1016/j.brainres.2009.11.067
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发表时间：
2010-02
期刊：
影响因子：
2.9
通讯作者：
A. Zhang;Yun Gao;Xiaoqin Zhong;Changshui Xu;Guiling Li;Shuangmei Liu;Jiari Lin;Xin Li;Yun Zhang;Hanqing Liu;Shangdong Linag
中科院分区：
文献类型：
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作者：
A. Zhang;Yun Gao;Xiaoqin Zhong;Changshui Xu;Guiling Li;Shuangmei Liu;Jiari Lin;Xin Li;Yun Zhang;Hanqing Liu;Shangdong Linag
神经性疼痛通常是持续性的，并且没有有效的治疗方法。初级感觉神经元中 P2X3 受体亚型的激活与神经性疼痛有关。阿魏酸钠（SF）是中草药的活性成分，具有抗炎活性。本研究观察SF对慢性缩窄性损伤(CCI)后大鼠P2X3受体介导的痛觉过敏的影响。测量机械撤退阈值（MWT）和热撤退潜伏期（TWL），并通过免疫组织化学和蛋白质印迹分析背根神经节（DRG）神经元中P2X3受体免疫反应性和蛋白的表达。在用SF治疗的CCI大鼠中，与用生理盐水治疗的CCI大鼠相比，MWT和TWL增加。 CCI后DRG神经元中P2X3受体的表达增加。在接受 SF 治疗的 CCI 大鼠中，DRG 神经元中 P2X3 受体的上调表达减少。 SF可能通过减少慢性神经病理性疼痛损伤期间由P2X3受体介导的初级传入神经元传递的疼痛来减轻CCI大鼠模型中的热和机械痛觉过敏。
Neuropathic pain is usually persistent and there is no effective treatment. Activation of P2X3receptor subtype in primary sensory neurons is involved in neuropathic pain. Sodium ferulate (SF) is an active principle from Chinese herbal medicine and has anti-inflammatory activities. This study observed the effects of SF on the hyperalgesia mediated by P2X3receptor of rats after chronic constriction injury (CCI). Mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) were measured and the expression of P2X3receptor immunoreactivity and protein in dorsal root ganglion (DRG) neurons was analyzed by immunohistochemistry and western blotting. In CCI rats treated with SF, the MWT and TWL were increased compared with CCI rats treated with normal saline. The expression of P2X3receptor in DRG neurons was increased after CCI. In CCI rats treated with SF, the up-regulated expression of P2X3receptor in DRG neurons was reduced. SF may reduce the thermal and mechanical hyperalgesia in CCI rat model by decreasing the pain transmitted by primary afferant neurons mediated by P2X3receptor during the chronic neuropathic pain injury.