The ziwi Promoter Drives Germ line-Specific Gene Expression in Zebrafish
The ziwi Promoter Drives Germ line-Specific Gene Expression in Zebrafish
复制标题
DOI：
10.1002/dvdy.22404
复制
发表时间：
2010-10-01
影响因子：
2.5
通讯作者：
Draper, Bruce W.
中科院分区：
文献类型：
--
作者：
Leu, David H.;Draper, Bruce W.
我们在这里描述了从斑马鱼生殖细胞特异性ziwi基因的5'端分离的4.8-kb序列的启动子活性的表征。我们表明，这个片段是足够的驱动异源基因表达，特别是在生殖细胞开始早在受精后7天，一个时间点，只有有丝分裂生殖细胞存在于性腺。在成年动物中，我们发现该片段足以驱动所有生殖细胞中的基因表达，其表达水平具有阶段特异性差异。在雌性中，EGFP表达在IB期卵母细胞中最高，但在减数分裂前生殖细胞以及II-IV期卵母细胞和成熟卵中检测到明显的表达。在男性中，EGFP在精原干细胞中表达最高，但在所有阶段（包括精子）中均检测到低水平。Developmental Dynamics 239：2714-2721，2010. (C)2010 Wiley-Liss，Inc.
We describe here the characterization of the promoter activity of a 4.8-kb sequence isolated from the 5' end of the zebrafish germ cell-specific ziwi gene. We show that this fragment is sufficient to drive heterologous gene expression specifically in germ cell starting as early as 7 days post-fertilization, a time point when only mitotic germ cells are present in the gonad. In adult animals, we find that this fragment is sufficient to drive gene expression in all germ cells with stage-specific differences in expression levels. In females, EGFP expression is highest in stage IB oocytes, but appreciable expression is detected in both pre-meiotic germ cells, as well stage II-IV oocytes and mature eggs. In males, EGFP expression is highest in spermatogonial stem cells, but low levels are detected in all stages, including spermatozoa. Developmental Dynamics 239:2714-2721, 2010. (C) 2010 Wiley-Liss, Inc.